Notch 1信号通路蛋白在不同程度颅脑损伤大鼠中的表达

The expression of Notch 1 signaling pathway proteins in rat models of different degree traumatic brain injury

目的:通过检测Notch 1信号通路的下游表达物Jagged 1、Hes 1在轻、中、重三种闭合性颅脑损伤(TBI)大鼠中的表达情况,探讨Notch 1信号通路在颅脑损伤中的作用。方法:利用液压冲击法模拟大鼠轻、中、重三种闭合性TBI模型;通过神经系统疾病严重程度评分(NSS)评估24 h后各组大鼠神经功能缺失情况;免疫组织化学及蛋白印迹方法检测三型TBI后脑皮质Notch 1信号通路下游表达因子Jagged 1及Hes 1的表达情况。结果:轻、中、重3组TBI模型中,NSS评分差异有统计学意义(P<0.05),造成的脑损伤越重,神经功能缺失情况越明显,说明模型建立成功;免疫组织化学及Western blot法检测结果显示轻、中、重三型TBI组的Hes 1和Jagged 1的表达水平均较对照组明显增高(P<0.05),且随着TBI程度的增高,该通路蛋白的表达量也在增加。结论:Notch 1信号通路可能参与了TBI的发展过程,可能成为TBI后治疗的新靶点。

Objective： By detecting Notch 1 signaling pathway proteins Jagged 1 and Hes 1 in light of heavy colsed craniocerebral injury. The role of Notch 1 signaling pathways in traumatic brain injury was explore and the new direction of treatment for closed craniocerebral injury was provide.Methods： Use lateral lfuid per-cussion method to make the light, medium and heavy closed craniocerebral trauma models. Groups of rats after 24 h of neurologic deifcits were evaluated through the nervous system disease severity score （NSS）, then use immunohistochemistry and Western blot methods were used to detect Notch 1 signaling pathway downstream factors Jagged 1 and Hes 1.Results： The NSS score difference among the TBI groups was statistically signiifcant （P〈0.05）, the brain contusion was heavier, the neurologic deifcits were more obvious, which means the model was established successfully. Immunohistochemistry and Western blot showed that in brain contusion the expres-sion of Hes 1 and Jagged 1 levels were signiifcantly higher than that the control group （P〈0.05）, and increased with the degree of brain injury, the expression of the proteins was increased.Conclusion： Notch 1 signaling path-way may be involved in the development process of craniocerebral injury, and may become the new target for therapy after traumatic brain injury.