成骨细胞刺激因子对去卵巢骨质疏松大鼠的治疗效果

Therapeutic effects of human osteoblast-stimulating factor on osteoporosis of ovariectomized rats

目的观察人成骨细胞刺激因子(OSF-1)对去卵巢骨质疏松大鼠的治疗效果。方法健康4月龄SD雌性大鼠12只,随机分为假手术组(SHAM组)、去势组(OVX组)、甲状旁腺激素组(PTH组)和OSF-1组,每组3只。SHAM组切除卵巢附近脂肪组织,其他各组切除双侧卵巢。术后10周,SHAM组和OVX组皮下注射生理盐水,0.2 m L/d,PTH组皮下注射PTH,20μg/(kg·d),OSF-1组皮下注射OSF-1,80μg/(kg·d)。术后21周,处死大鼠,分离子宫并称重,分离股骨,显微CT扫描干骺端。结果 OVX组子宫重和骨量较SHAM组显著降低(P<0.01),骨小梁微结构明显破坏。与OVX组相比,PTH组和OSF-1组的骨密度、骨体积分数、骨小梁数量显著增加(P<0.01),骨小梁分离度和模式因子显著下降(P<0.01,P<0.05),骨小梁微结构更紧密。PTH组较OSF-1组骨密度、骨体积分数、骨小梁数量显著增加(P<0.01),骨小梁模式因子显著下降(P<0.01)。结论皮下注射OSF-1[80μg/(kg·d)]可有效增加去卵巢大鼠的骨量,改善骨小梁微结构。

Objective To investigate the therapeutic effects of human osteoblast-stimulating factor（ OSF-1） on the osteoporosis of ovariectomized rats. Methods A total of 12 female rats aged 4 months were randomly divided into 4groups： SHAM group,OVX group,human parathyroid hormone group（ PTH group） and OSF-1 group. A small piece of adipose tissue was cut off from rats in SHAM group; bilateral ovariectomy was conducted in rats in the other groups.All rats were intervened per day separately since 10 weeks after the ovariectomy： SHAM and OVX groups received subcutaneous injection of 0. 2 m L / d sodium chloride solution, PTH group was given subcutaneous injection of20 μg /（ kg·d） PTH,and OSF-1 group was injected subcutaneously with 80 μg /（ kg · d） OSF-1. Pharmacological agents were administrated consecutively for 11 weeks,then all rats were sacrificed. Uterus was removed and weighed.Femur was removed and cleaned of adhering muscles and connective tissues. M icrocomputed tomography（ micro-CT）w as used to scan proximal femur metaphysis. Results Uterus weight and bone mass in OVX group were significantly lower than those in SHAM group（ P〈0. 01）,and the microstructure of bone trabecula was destructed in OVX group.Compared with OVX group,bone morphometric parameters in PTH and OSF-1 groups show ed significant increase on bone mineral density（ BM D）,BV / TV,Tb. N（ P〈0. 01）,while Tb. Sp and TBPf were lower（ P〈0. 01,P〈0. 05）,and bone trabecula were more conspicuous in PTH and OSF-1 groups. The parameters of BM D,BV / TV and Tb. N in PTH group were higher than those in OSF-1 group（ P〈0. 01）,and TBPf was lower than that in OSF-1 group（ P〈0. 01）. Conclusion Subcutaneous injection of 80 μg /（ kg ·d） OSF-1 can increase bone mass and improve bone microstructure in ovariectomized rats.