益气活血养阴解毒方对放射性肺损伤中TGF-β1、TNF-α表达的影响

Effect of Yiqihuoxue-Yangyinjiedu recipe on expressions of TGF-β1 and TNF-α in radiation-induced lung injury

目的研究益气活血养阴解毒方对放射性肺损伤中转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)表达的影响,探讨其对放射性肺损伤的防治作用及机制。方法将小鼠随机分为对照组(n=21)、中药组(n=21)、照射组(n=21)、治疗组(照射+中药,n=21),用直线加速器对后两组小鼠全肺进行单次照射(12 Gy),分别于照射后1、24、72 h和2、4、8、24周处死小鼠,取标本进行组织学观察、RT-q PCR及免疫组化检测,观察小鼠组织学变化及不同时间点的TGF-β1、TNF-α的表达。结果益气活血养阴解毒方可以改善放射性肺损伤。照射组TGF-β1、TNF-α的m RNA及蛋白水平表达均高于对照组及中药组(P<0.05);除照射后1、24 h外,治疗组TGF-β1、TNF-α在其余各时间点的表达水平均低于照射组(P<0.05)。结论益气活血养阴解毒方具有抑制照射刺激下TGF-β1、TNF-α的表达、减轻放射性肺损伤的作用,其机制与抑制TGF-β1、TNF-α的表达有关。

Objective To investigate the influence of traditional Chinese medicine,Yiqihuoxue-Yangyinjiedu recipe,on transforming growth factor-β1（ TGF-β1） and tumor necrosis factor-α（ TNF-α） in lung tissues of mice with radiation-induced lung injury,and to explore the effects and action mechanism of Yiqihuoxue-Yangyinjiedu recipe on preventing and treating radiation-induced lung injury. Methods M ice were randomly divided into control group（ n = 21）,drug group（ n = 21）,radiation group（ n = 21）,and treatment group（ radiation ＋ drug,n = 21）. The latter two groups were irradiated at whole thorax with a dose of 12 Gy. The pathology of radiation-induced lung injury was monitored.The expressions of TGF-β1 and TNF-α were analyzed by RT-q PCR and immunohistochemistry at 1,24,72 h and 2,4,8,24 week post-irradiation. Results The results of histology demonstrated that Yiqihuoxue-Yangyinjiedu recipe treatment ameliorated radiation-induced lung injury. Expressions of TGF-β1,TNF-α m RNA and protein in radiation group were higher than those in control group and drug group（ P〈0. 05）. Except at 1 h and 24 h,expressions of TGF-β1 and TNF-α in treatment group at each time point were lower than those in radiation group（ P〈0. 05）. Conclusion Yiqihuoxue-Yangyinjiedu recipe can inhibit the expressions of TGF-β1 and TNF-α,and may play an important role in preventing and treating radiation-induced lung injury,which may be related to inhibit the expressions of TGF-β1 and TNF-α.