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膀胱癌T24耐药株的构建及其特性分析

Establishment of a drug-resistant bladder cnacer cell line T24/MMC and its biological characteristics
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摘要 目的建立膀胱癌T24耐药细胞株,并观察其相关生物学特性。方法采用丝裂霉素(MMC)浓度梯度持续递增法诱导建立膀胱癌T24耐药株,并从生长能力、克隆形成能力、迁移和侵袭能力、微球体形成能力等方面进行比较。结果膀胱癌T24耐药株与亲本细胞相比,其增殖速度明显高于亲本T24细胞;耐药株的克隆形成率、迁移和侵袭能力、成球能力均提高。结论低浓度丝裂霉素持续增量法可诱导形成膀胱癌T24耐药细胞株,且耐药株T24/MMC恶性程度更高,癌干细胞被富集。 Objective To establish a human drug-resistant bladder cancer cell line（T24 / MMC） and to investigate its characteristics.Methods Human bladder cancer cell line T24 was established using gradually increased concentration of mitomycin（MMC） in culture.Basic characteristics of both the parental cell line T24 and the drug-resistant cell line T24 / MMC were analyzed and compared,including the ability of proliferation,colony formation,mobility and invasion,sphere forming ability.Results Compared with T24,the T24 / MMC cell grows quickly,and the ability of proliferation,colony formation,mobility and invasion,stemness gene,sphere forming ability increased.Conclusion Gradually increased concentration of MMC can induce drug-resistant bladder cancer cell line,and it was enriched of cancer stem cells.
作者 邱敏 曹胜男 宁志丰 甘亚平 宋铁山 刘复兴
机构地区 湖北科技学院基础医学院
出处 《解剖学研究》 CAS 2015年第3期214-216,220,共4页 Anatomy Research
关键词 膀胱癌 T24 耐药株 肿瘤干细胞 Bladder cancer T24 Drug-resistant cancer cell line Cancer stem cells
分类号 R737.14 [医药卫生—肿瘤]
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参考文献10

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