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IL-22及IL-22siRNA对HaCaT细胞分泌HB-EGF的影响

Effects of IL-22 and IL-22 siRNA on the Expression of Heparin-binding Epidermal-growth-factor-like Growth Factor in HaCaT Cells
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摘要 目的研究IL-22及IL-22siRNA对HaCaT细胞肝素结合表皮生长因子样生长因子（heparin—bindingepidermalgrowthfactor-1ikegrowthfactor，HB—EGF）表达的影响。方法用12．5～100μg／L的IL-22和IL-22siRNA干预处理HacaT细胞，继续培养24h后，分别用实时荧光定量RT—PCR和Westernblot法、ELISA法检测IL-22及IL-22siRNA对HaCaT细胞HB—EGFmRNA和蛋白的影响。结果不同浓度的IL-22干预处理后，HB-EGF的mRNA和蛋白水平均较对照组升高，且随着IL-22浓度的升高，其促HB-EGF的表达作用逐渐增强，与对照组相比，差异具有统计学意义（P〈0．05）。IL-22siRNA处理组的HB-EGFmRNA和蛋白水平较对照组低，与对照组相比，差异具有统计学意义（P〈0．05）。结论IL-22可剂量依赖促进HB-EGF的表达，IL-22siRNA可抑制HB-EGF的表达。 Objective To investigate the effects of interleukin-22 （IL-22） and IL-22 siRNA on HB-EGF expression in HaCaT cells. Methods After being treated with 12. 5 - 100μg/L IL-22 and IL-22 siRNA for 2gh,total RNA and total protein were collected. Level of HB-EGF mRNA was examined by real-time quantitative RT-PCR, and protein level of HB-EGF was detected by western blot and ELISA. Results Compared with the control, both mRNA and protein level of HB-EGF were elevated with the increase concentration of IL-22 treatment, and the promoting function gradually enhanced. However, the expression of HB-EGF decreased significantly after IL-22 siRNA exposure. The difference were statistically significant in IL-22 and IL-22 siR- NA. Conclusion IL-22 treatment can dose-dependently induce HB-EGF expression, IL-22 siRNA can in- hibit HB-EGF expression.
作者 许文娟 刘欣欣 罗素菊 郑焱 李燕 刘全忠
机构地区 天津医科大学总医院皮肤科 西安交通大学第二医院皮肤科
出处 《中国皮肤性病学杂志》 CAS CSCD 北大核心 2015年第7期663-666,共4页 The Chinese Journal of Dermatovenereology
基金 国家自然基金资助项目(81201231 81371732)
关键词 IL-22 HB-EGF HACAT细胞 Interleukin-22 HB-EGF HaCaT cells
分类号 R758.63 [医药卫生—皮肤病学与性病学]
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参考文献8

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二级参考文献9

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中国皮肤性病学杂志
2015年 第7期

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