全自动生化分析仪测定尿碘方法的研究

A method for determination of urinary iodine by automatic biochemical analyzer

目的建立全自动生化分析仪测定尿中碘含量的方法，分析该方法在碘缺乏病监测中的适用性。方法结合全自动生化分析仪的硬件条件，应用低砷试剂进行尿中碘含量测定，并进行相应的线性范围、检测限、精密度、加标回收、抗干扰和方法对比等验证实验。结果碘标准曲线线性范围为0～300μg／L，方法检测限为1．57μg／L（取样量为600μl）。精密度：测定尿中碘含量为（84．8±1．1）、（156．5±1．3）、（227．7±2．4）μg／L尿样时，相对标准差（RSD）分别为1．30％、0．83％、1．05％；准确度：对低、中、高3种碘含量尿样加标平均回收率分别为98．0％、100．1％、98．6％，总平均回收率为98．9％。对国家尿碘标准物质进行检测，结果满足准确度的判断标准，此方法准确可靠。方法对比试验，全自动生化分析仪与砷铈催化分光光度法（国标法）各检测24份8—10岁儿童尿样，经配对t检验，差异无统计学意义（t=0．35，P〉0．05）；与国标法比较，全自动生化分析仪亚砷酸的使用量仅为其2％（mg／份：0．50比25．00）。结论全自动生化分析仪检测尿碘，操作简单，对环境要求低，检测线性范围合理；使用自配标准溶液和试剂，降低检测的试剂成本；极大降低砷的使用，减少了环境污染和对检测人员健康的危害。该方法实现了自动化检测，检测速度快，适用于碘缺乏病监测中大批量尿碘样品的检测。

Objective To establish a method for determination of urinary iodine by an automatic biochemical analyzer, and to analysis the applicability on the detection of iodine deficiency disorders. Methods An automatic biochemical analyzer was used to determine urinary iodine content. Linear range, detection limit, precision, recovery and so on were studied. Results The linear range was 0 - 300 μg/L; the detection limit was 1.57 μg/L （600 μl sample）; the relative standard deviations （RSD） were 1.30%, 0.83% and 1.05% when measuringurine samples with iodine concentrations of（84.8 ±1.1） （156.5 ± 1.3） and （2;27.7 ± 2.4）μg/L, respectively; the average recovery was 98.0%, 100.1% and 98.6%,and the total average recovery Was 98.9%, when measuring urine samples containing three different iodine concentrations. The test results of standard material were all within the required ranges. The difference of measuring 24 urine samples in the field by this method and the standard method was not statistically significant by .paired t test （t = 0.35, P 〉 0.05）. Compared with standard method, the amount usage of arsenic trioxide was reduced to 2% （0.50 to 25.00 mg per sample）. Conclusions The method has the advantages of simple operation, low requirement for environment, and the linear rang of detection is reasonable. Using this method, the usage of highly toxic reagent can be greatly reduced, as well as the risk of harm to personnel health and the level of environment pollution. The standard solution and reagents can be prepared by ourselves, which greatly reduces the costs. With short reaction time, high detection speed and measuring automatically for large numbers of samples , this method for determination of urinary iodine by an automatic biochemical analyzer can be applied in monitoring of iodine deficiency disorders.