补骨脂素抗菌双相陶瓷骨的制备及性能检测

Preparation and performance of psoralen antibiotic biphasic ceramic bone

背景:近年来中药在骨组织工程中应用的研究在国内处于起步阶段,尚未见到补骨脂素在骨组织工程支架材料中应用的研究报道。目的:通过真空复合方法制备出补骨脂素抗菌双相陶瓷骨,检测材料的理化性能、抗菌性和细胞相容性。方法:将猪椎骨经复合焦磷酸钠两次低温煅烧制备双相陶瓷骨,将双相陶瓷骨置于壳聚糖/补骨脂素复合溶液中,制备补骨脂素抗菌双相陶瓷骨。扫描电镜观察补骨脂素抗菌双相陶瓷骨的表面形貌,并检测其抗压强度;采用微生物法检测补骨脂素抗菌双相陶瓷骨对大肠杆菌、金黄色葡萄球菌的抑制效果;将补骨脂素抗菌双相陶瓷骨与小鼠成骨细胞MC3T3-El共培养4,8 d,扫描电镜观察细胞在材料表面的附着与增殖。结果与结论:补骨脂素抗菌双相陶瓷骨具有天然的孔隙结构,骨小梁、小梁间隙和骨内管腔系统同时存在,抗压强度为(4.69±0.50)MPa,其对大肠杆菌、金黄色葡萄球菌均有较强的抑制作用,抑菌效果明显;小鼠成骨细胞MC3T3-El可在补骨脂素抗菌双相陶瓷骨上很好的附着,保持良好的状态和功能并快速增殖,说明补骨脂素抗菌双相陶瓷骨具有良好的细胞相容性。

BACKGROUND： In recent years, studies on the application of traditional Chinese medicine in bone tissue engineering are still at the initial stage in China. Reports about psoralen antibiotic biphasic ceramic bone have not been seen in bone tissue engineering research. OBJECTIVE： To fabricate the psoralen antibiotic biphasic ceramic bone by vacuum evaporator and to observe the physicochemical properties, antibiotic activity and biocompatibility of the material. METHODS： Biphasic ceramic bone was prepared by twice low-temperature calcining pig vertebrae combined with sodium pyrophosphate, and then the ceramic bone was immersed in chitosan/psoralen compound solution to prepare psoralen antibiotic biphasic ceramic bone. The surface morphology was observed by scanning electron microscope, and the compressive strength was tested. Antibiotic activity of the psoralen antibiotic biphasic ceramic bone on Escherichia coli and Staphylococcus aureus was tested by microbiological methods. Mouse osteoblasts MC3T3-E1 were co-cultured with the psoralen antibiotic biphasic ceramic bone for 4 and 8 days, and the cell adhesion and proliferation on the scaffold surface were observed under the scanning electron microscope. RESULTS AND CONCLUSION： Psoralen antibiotic biphasic ceramic bone had natural pore structure with the trabecular bone, trabecular space and lumen system and exerted great antibiotic effect on Escherichia coli and Staphylococcus aureus. The compressive strength was（4.69±0.50） MPa. Mouse osteoblasts MC3T3-E1 M could be adherent to the psoralen antibiotic biphasic ceramic bone and proliferate rapidly, suggesting the psoralen antibiotic biphasic ceramic bone has good cytocompatibility.