不同株系菠萝蜜染色体倍性及基因组大小分析

Determination and analysis of ploidy and genome size of different jackfruit lines

【目的】为了解菠萝蜜基因组特点,研究测定了10份菠萝蜜株系的倍性和基因组大小。【方法】分别以番茄和‘妃子笑’荔枝细胞核DNA含量为内标,Otto为提取液,采用流式细胞术(FCM)测定经碘化丙啶(PI)染色的内标和菠萝蜜细胞核混合样品发出的PI荧光强度,通过比较内标和菠萝蜜DNA峰值的荧光均值比值关系,计算出不同株系菠萝蜜的倍性和基因组大小。【结果】所测的10份菠萝蜜株系都为二倍体,干苞菠萝蜜株系的基因组在(1 243.75±9.21)^(1 540.63±3.55)Mb,湿苞菠萝蜜株系的基因组约为(1 902.87±8.08)Mb。【结论】不同菠萝蜜株系之间基因组大小存在显著差异,且湿苞株系大于干苞株系。

【Objective】To study the character of genome sequence,evolvement biology and the screeningof functional genes of jackfruit,determination of the genome size of jackfruits was an preliminary task.【Methods】Tomato and litchi‘feizixiao＇were served as internal references,and Otto was extract. The flu-orescence intensities of the internal reference and jackfruit were measured by flow cytometry（ FCM） afterthe mixed nucleus samples dyed by propidium iodide（PI）. Comparing the multiple relationships betweenthe two peak values,the polidy level and genome size was calculated for different jackfruit lines.【Re-sults】10 selected jackfruit lines were diploid. The hard jackfruit genome size range from（1 243.75±9.21）Mb to（1 540.63±3.55）Mb,while the soft jackfruit genome size was about（1 902.87±8.08）Mb.【Conclu-sion】The genome sizes varied in different jackfruit lines,and the Nuclear DNA contents of soft lineswere much greater than that of hard lines.