硫化氢对盐胁迫条件下大豆抗氧化酶活性的影响

Effects of Hydrogen Sulfide Alleviates Salt Stress in Soybean( Glycine max) Antioxidative System

以南农1138-2为材料,采用不同浓度(0,0.20,0.40,0.60和0.80 mmol·L-1)的H2S供体Na HS溶液喷施在0.08 mol·L-1的Na Cl溶液胁迫下的大豆植株上,分析大豆从V2至V4期生长和同工酶活性的影响。结果表明:0.08mol·L-1的Na Cl溶液能有效抑制大豆生长。H2S的供体Na HS溶液喷施对盐胁迫有一定的缓解作用,0.20和0.40mmol·L-1的Na HS处理的子叶等级显著高于阳性对照(0.00 mmol·L-1Na HS处理),0.40 mmol·L-1的Na HS处理有最大的根冠比。0.40 mmol·L-1的Na HS处理下的V3和V4期大豆叶片中的过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性虽然比阴性对照低,但比其余处理都高,并都显著高于阳性对照。并且在V3期大豆的子叶等级与抗氧化酶活性成正相关关系。POD和SOD的同工酶在Na HS处理下谱带都发生了一定程度的改变。

Nannong1138-2 was used as test material,0. 08 mol·L- 1Na Cl stressed soybeans were sprayed different concentration Na HS（ 0,0. 20,0. 40,0. 60 and 0. 80 mmol·L- 1）. The effects of growth and isozymes activity from V2 to V4 periods were analyzed by different treatments. The results showed that growth of soybean was inhibited by 0. 08 mol·L- 1Na Cl solution. H2 S donor Na HS alleviates Na Cl-induced stress. Treatments of 0. 20 and 0. 40 mmol·L- 1Na HS significantly increased the ranks of cotyledon than positive control and treatment of 0. 40 mmol·L- 1Na HS had the highest ratio of root shoot. The activities of POD,SOD and CAT of treatment of 0. 4 mmol·L- 1Na HS was lower than negative control but higher than others and significantly higher than positive control. The ranks of cotyledon were positively correlated with activities of antioxidative.Na HS treatments caused changes of the band number of POD and SOD.