摘要
本研究建立了一种用高效液相色谱测定婴儿配方奶粉中乳铁蛋白的方法。色谱条件：色谱柱为Hi Trap Heparin肝素琼脂糖亲和柱（0.7×2.5cm,1m L）,流动相A为50mmol/L的Na2HPO4溶液（p H7.00）,流动相B为50mmol/L的Na2HPO4＋1.0mol/L的Na Cl溶液（p H7.00）,检测波长为230nm。结果表明：乳铁蛋白的检出限为3.0mg/100g,回收率在86%~99%之间,相对标准偏差（RSD）为3.4%。用此方法检测婴儿配方奶粉中乳铁蛋白,操作简单,结果准确可靠,精密度高,重现性好。
A method was established for the determination of lactoferrin in infant formula milk powder by high performance liquid chromatography（HPLC）. The chromatographic condition was adopted as follows： Hi Trap Heparin column （0.7×2.5cm, lmL）, a diode-array detector （DAD） at the wavelength of 230nm, and the mobile phase of 50mmol/L dibasic sodium phosphate solution with 1.0 mol/L sodium chloride solution .The result indicated the recoveries of lactoferrin was ranging from 86%- 99%, the detection limit was 3.0mg/100g, and the value of relative standard deviation （RSD） was 3.4%. The method has the advantages of simple operation, precise result, high precision and good repeatability.
出处
《中国奶牛》
2015年第13期49-52,共4页
China Dairy Cattle
基金
石家庄市科技支撑计划项目(编号:141171201A)
