不同来源北冬虫夏草活性成分差异及其对小鼠免疫功能的影响

Determination of Active Components in Cordyceps militaris from Different Sources and Their Impacts on Immune Function of Mice

目的:探讨不同来源北冬虫夏草主要活性成分的差异,并评价其调节小鼠免疫功能的能力。方法:使用高效液相色谱法测定不同来源北冬虫夏草虫草素和腺苷的含量;通过测定小鼠免疫器官指数以及碳廓清实验和脾淋巴细胞增殖实验研究其对小鼠免疫功能的影响。结果:虫草素含量由高到低依次为蚕蛹虫草3.68 mg/g、小麦虫草2.86 mg/g、大米虫草2.63 mg/g、头状虫草0.95 mg/g;腺苷含量由高到低依次为蚕蛹虫草1.11 mg/g、小麦虫草0.79 mg/g、头状虫草0.64 mg/g、大米虫草0.094 mg/g。碳廓清指数和脾淋巴细胞增殖能力研究结果表明4种虫草均有提高小鼠免疫活性的功效,与空白组相比差异极显著,蚕蛹虫草和小麦虫草功效最佳;饲喂4种虫草的小鼠免疫器官指数与空白组相比差异显著。结论:不同来源北冬虫夏草的虫草素和腺苷含量有所差异,蚕蛹虫草和小麦虫含量最高,其提高小鼠免疫功能的能力也最强。

Purpose：To comparatively analyze active components in Cordyceps militaris from different sources and investigate the effects of Cordyceps militaris from different sources on the immune function of mice.Methods：High performance liquid chromatography（HPLC） was used to determine the contents of cordycepin and adenosine in four kinds of Cordyceps militaris,and their immune-regulatory effects on mice were evaluated by carbon clearance rate,immune organ indexes and proliferation capacity of spleen lymphocytes.Results：Silkworm chrysalis Cordyceps militaris,wheat Cordyceps militaris,rice Cordyceps militaris and Cordyceps capitata contained 3.68,2.86,2.63 and 0.95 mg/g cordycepin,and 1.11,0.79,0.64 and 0.094 mg/g adenosine,respectively.Based on the results of carbon clearance rate,proliferation capacity of spleen lymphocytes and immune organ indexes,all the four kinds of Cordyceps militaris could increase the immune function of mice when compared with the control group.Meanwhile,silkworm chrysalis Cordyceps militaris and wheat Cordyceps militaris were much better in improving the immune function than those from two other sources.Conclusions：The contents of cordycepin and adenosine were significantly different among Cordyceps militaris from different sources,and Cordyceps militaris from silkworm chrysalis and wheat had higher contents of cordycepin and adenosine,and exerted more potent immune-regulatory effects on mice.