南方根结线虫侵染对不同抗性番茄砧木渗调物质及羟脯氨酸含量的比较

The effects of Meloidogyne incognitainfection on osmolyte and hydroxyproline levels in tomato rootstock seedlings with different resistance

为探讨番茄砧木对南方根结线虫的抗性生理机制,以高感砧木Ls-89与高抗砧木坂砧2号为试材,采用盆栽人工接种线虫二龄幼虫法,研究了南方根结线虫侵染对不同抗性番茄砧木幼苗渗透调节物质及细胞壁羟脯氨酸含量的影响。研究表明,未接种南方根结线虫的番茄砧木幼苗,其根系与叶片可溶性糖、可溶性蛋白、脯氨酸等渗透调节物质与细胞壁羟脯氨酸含量变化并未因品种抗性不同而有显著差异(P>0.05);接种南方根结线虫后,渗透调节物质含量与细胞壁羟脯氨酸含量变化规律基本一致,均呈周期性升高,且坂砧2号升幅显著高于Ls-89(P<0.05)。据此认为,可溶性糖、可溶性蛋白、脯氨酸、细胞壁羟脯氨酸等物质的含量变化均与番茄砧木幼苗对南方根结线虫的抗性密切相关,可作为评判植株抗性的综合指标。

In order to clarify the physiological resistance mechanism of tomato rootstock to Meloidogyne incognita ,both a highly susceptible rootstock variety Ls-89 and a highly resistant rootstock variety Banzhen No.2 were planted in pots and inoculated with second instar larvae to study the effects of M.incognita infection on osmolyte levels and cell wall hydroxyproline contents of these differentially resistant tomato rootstock varieties.Without M.incognita inoculation,there was no significant difference （P 〉0.05）between seedlings of the two varieties in concentrations of osmoregulation substances,including soluble sugar,soluble protein,proline and cell wall hydroxyproline.After inoculation,the concentrations of osmoregulation substances and cell wall hydroxyproline contents were increased in roots and leaves in seedlings of the two varieties,with the responses in Banzhen No.2 being significantly higher （P 〈0.05）than those of Ls-89.These results indicate that changes in soluble sugar,soluble protein,proline and cell wall hydroxyproline concentrations are closely associated with the resistance of tomato rootstock seedlings to M.incognita,and could be used as general indices of plant resistance elevation.