摘要
以奶牛乳腺上皮细胞为模型,研究丝裂原活化蛋白激酶激酶激酶9(MAP3K9)对细胞增殖和β-酪蛋白(CSN2)合成的影响。对细胞进行蛋氨酸刺激,检测细胞增殖及细胞中CSN2和MAP3K9的表达。过表达或抑制MAP3K9,检测细胞增殖及细胞中CSN2,MAPK1,p-MAPK1,m TOR和p-m TOR的表达。过表达MAP3K9且抑制MAPK1,检测细胞增殖与细胞中m TOR、p-m TOR和CSN2的表达。结果表明:蛋氨酸刺激使细胞增殖及细胞中CSN2和MAP3K9的表达显著增加(p<0.01);MAP3K9过表达或抑制时,细胞增殖及细胞中CSN2,MAPK1,p-MAPK1,m TOR和p-m TOR的表达均显著增加或降低(p<0.01),但抑制MAPK1时,过表达MAP3K9不能使细胞增殖及细胞中m TOR,p-m TOR和CSN2的表达增加。结果表明,作为一个调节因子,MAP3K9能通过激活细胞中的MAPK1来激活m TOR通路,正向调控奶牛乳腺上皮细胞增殖与乳蛋白合成。
The effects ofmitogen-activated protein kinase kinase kinase 9 (MAP3K9) on proliferation and β-casein (CSN2) synthesis in bo- vine mammary epithelial cells (BMECs) was investigated. The proliferation and expression of CSN2 and MAP3K9 of BMECs stimulated withmethionine (Met) were tested. After treated with MAP3K9 overexpression or inhibition, the proliferation and expression of CSN2, MAPK1, p-MAPK1, roTOR and p-mTOR of BMECs were detected. Treated with MAP3K9 overexpression and MAPK1 inhibition, the proliferation and expression of CSN2, roTOR and p-roTOR of BMECs were determined. The results showed that: the proliferation and ex- pression of CSN2 and MAP3K9 were significant increased (P〈0.01) after treated with Met stimulation. After MAP3K9 overexpression and in- hibition, the proliferation expression of CSN2, MAPK1, p-MAPK1, mTOR and p-mTOR of BMECs were significant increases and reduced (P〈0.01), respectively. But after MAPK1 inhibition, MAP3K9 overexpression could not increase the proliferation and expression of CSN2, mTOR and p-mTOR of BMECs. The study suggests that as a regulator, MAP3K9 can activate mTOR pathways by activating MAPK1, and positively regulate the proliferation and expression of CSN2 in BMECs.
出处
《中国乳品工业》
CAS
CSCD
北大核心
2015年第7期16-20,61,共6页
China Dairy Industry