葡萄糖激酶调节蛋白基因rs1260326多态性与新疆维吾尔族人群2型糖尿病的相关性研究

Relationship Between the Polymorphism of GCKR Gene rs1260326 and Type 2 Diabetes in Uygur in Xinjiang

目的探讨葡萄糖激酶调节蛋白(GCKR)基因rs1260326多态性与新疆维吾尔族2型糖尿病(T2DM)的关系。方法选取2012年6月—2013年3月新疆医科大学第一附属医院确诊为T2DM的维吾尔族患者1 000例为T2DM组,同期选取该院的体检健康者1 008例为对照组。收集所有受试者身高、体质量、臀围、腰围(WC)、收缩压(SBP)、舒张压(DBP)等一般临床资料,计算体质指数(BMI)、腰臀比(WHR)。检测并比较其生化指标〔总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、空腹血糖(FPG)〕。采用Sequenom Mass ARRAY系统对GCKR基因rs1260326位点进行基因分型,比较T2DM组和对照组GCKR基因rs1260326位点基因型分布及等位基因频率差异;采用Logistic回归分析GCKR基因rs1260326多态性与T2DM的关系。结果 T2DM组BMI、WHR、WC高于对照组(P<0.05);两组SBP、DBP比较,差异无统计学意义(P>0.05)。T2DM组TC、FPG高于对照组,HDL-C、LDL-C低于对照组(P<0.05);两组TG比较,差异无统计学意义(P>0.05)。两组GCKR基因rs1260326位点基因型分布均符合Hardy-Weinberg遗传平衡定律(P>0.05),说明两组样本具有群体代表性。T2DM组与对照组rs1260326位点基因型分布、rs1260326位点等位基因频率比较,差异无统计学意义(P>0.05)。Logistic回归分析结果表明,GCKR基因rs1260326隐性遗传模式是T2DM的影响因素(P<0.05)。多因素Logistic回归分析结果显示,入选主效应模型的变量有性别、WHR、TC、TG、HDL-C、GCKR基因rs1260326隐性遗传模式(P<0.05)。结论在新疆维吾尔族中,GCKR基因rs1260326多态性与T2DM相关,TT基因型可能是新疆维吾尔族人群T2DM发生的保护因素。

Objective To investigate the relationship between the polymorphism of glucokinase regulator （GCKR） gene rs1260326 and type 2 diabetes （T2DM） in Uygur in Xinjiang. Methods 1 000 Uygur patients who were diagnosed with T2DM in the First Affiliated Hospital of Xinjiang Medical University from June 2012 to March 2013 were selected as the T2DM group. Another 1 008 healthy people who received physical examination in the same hospital in the same time period were enrolled as the control group. The general data of the subjects were collected,, including height, body mass, hipline, waist circumference （WC）, systolic blood pressure （SBP） and diastolic blood pressure （DBP） . Then the BMI and WHR of the subjects were calculated. Biochemical indicators were also tested and compared, including TC, TG, HDL- C, LDL- C and FPG. Scquenom Mass ARRAY system was employed to conduct genetic typing on the locus of GCKR gene rs1260326. The two groups were compared in the genotype of GCKR gene rs1260326 locus and the distribution frequency of GCKR gene rs1260326 allele. Logistic regression analysis was conducted to analyze the relationship between the polymorphism of GCKR gene rs1260326 locus and T2DM. Results The T2DM was higher （ P 〈 0. 05 ） than the control group in BMI, WHR and WC. The two groups were not significantly different （P 〉0. 05） in SBP and DBP. The T2DM group was higher （P 〈0. 05） in TC and FPG and lower （P 〈 0. 05） in HDL - C and LDL - C than the control group. The two group were not significantly different （ P 〉 0. 05 ） in TG. The frequency distributions of the genotype of GCKR gene rs1260326 locus of the two groups accorded with the Hardy - Weinberg equilibrium, which indicated that the samples of the two groups were representative of the population. The T2DM group was not significantly different （ P 〉 0.05 ） in the genotype of rs1260326 locus and rs1260326 locus allele from the control group. The Logistic regression analysis showed that the recessive genetic model of GCKR gene rs1260326 was the influencing factor for T2DM （P 〈 0.05 ） . The muhivariate Logistic regression analysis showed that the variables that entered the main effect model included gender, WHR, TC, TG, HDL- C and the recessive genetic model of GCKR gene rs1260326 （P 〈 0.05 ） . Conclusion Among Uygur in Xinjiang, the polymorphism of GCKR gene rs1260326 is correlated with T2DM, and TY genotype may be the protective factor for the occurrence of T2DM.