山楂酸对糖皮质激素诱发骨质疏松大鼠的干预作用研究

Effect of maslinic acid on glucocorticoid-induced osteoporosis in rats

目的研究山楂酸对糖皮质激素造成大鼠骨质疏松的干预作用及其作用机制。方法 60只SD大鼠分为6组,正常组、模型组、对照组(灌胃给予碳酸钙375 mg·kg-1·d-1及维生素D 350 U·kg-1·d-1)及3个剂量实验组(灌胃给予山楂酸100,50,25 mg·kg-1·d-1),共给药12周。生化试剂盒测定血清中的钙、磷的含量、骨钙素含量和抗酒石酸酸性磷酸酶活性,双能X线骨密度测定仪测定骨密度。结果与正常组相比,泼尼松可导致骨无机质钙盐和有机质羟脯胺酸下降,骨密度下降。山楂酸能对抗由泼尼松引起的上述异常,在增加骨密度方面优于对照组。结论山楂酸能有效预防糖皮质激素造成的大鼠骨质疏松。

Objective To analyze the effect of maslinic acid on glucocorticoid-induced osteoporosis in rats. Methods Animal experiments were performed to analyze the effect of maslinic acid on the osteoporosis. All the rats were randomly separated into six groups： normal group,model group,control group and three doses test groups（ maslinic acid,100,50,25 mg · kg-1·d-1）. The effect of maslinic acid on the administration of osteoporosis was evaluated through detecting the relevant biochemical markers including calcium,phosphorus,osteocalcin and tartrate-resistant acid phosphatase（ TRAP） activities by reagent Kits. The bone density was evaluated by using dual energy X ray absorptionmetry.Results Compared with model group,the maslinic acid has the effect on osteoporosis through adjusting the absorption and excretion of calcium.The bone loss and damage of the tibia and the femur of rats in maslinic acid groups can be significantly ameliorated and the ability to resist the fracture was improved effectively. Conclusion The maslinic acid can ameliorate the cancellous variation in glucocorticoid induced osteoporosis in rats.