孟根乌森乌日乐对小鼠的急性毒性研究

Study of acute toxicity of Meng gen wu sen ri le in mice

目的了解孟根乌森乌日乐的急性毒性作用剂量及给药后的急性毒性反应和死亡分布情况,确定孟根乌森乌日乐的半数致死量（LD50）。方法用孔氏综合法（改进寇氏法）分为14.30,9.28,6.04,3.92,2.55,1.66 g·kg-16个剂量组,以0.4 m L/10 g的量灌胃给药1次。实验后观察14 d,记录体重变化及不良反应情况。结果孟根乌森乌日乐小鼠半数致死量为5.1597 g·kg-1（95%CI：3.6652-7.2637 g·kg-1）。14 d内未出现明显不良反应症状且体重有增长趋势。结论孟根乌森乌日乐的急性毒性实验的半数致死量为临床用药量的100倍,提示单次口服较为安全。

Objective To investigate the study of acute toxicity of mongolian medicine Meng gen wu sen ri le,to understand the dose of acute toxicity and distribution and death of acute toxicity after the administration,to determine lethal dose 50（ LD50） of Meng gen wu sen ri le.Methods According to the results of preliminary experiments,using Conn synthesis method（ modified Karber method）, divided into six groups dosage are 14. 30,9. 28,6. 04,3. 92,2. 55,1. 66 g·kg-1,after experiments observing 14 day,to record the change of the weight and adverse reaction. Results LD50 of Meng gen wu sen ri le is 5. 1597g·kg-1（ 95% CI： 3. 6652-7. 2637 g·kg-1）. In 14 days,it does not appear obvious symptoms of adverse reaction and body weight has growth trends. Conclusion According to the results of experiments,LD50 of Meng gen wu sen ri le is 100 times of the clinical dosage,tip single oral is safer.