摘要
以冷冻结晶法对藻油中二十二碳五烯酸(DPA)和二十二碳六烯酸(DHA)甘油酯进行了分离富集研究,并采用气相色谱-质谱联用对产物中脂肪酸组成及含量进行分析。实验确定的最佳工艺条件为:以丙酮为溶剂,藻油与溶剂体积比1∶6,结晶温度-40℃,结晶时间8 h。在最佳条件下,富集藻油中DPA含量从11.65%提升至14.36%,DHA含量从44.64%提升至57.47%。
The enrichment of docosapentenoic acid （DPA） and docosahexaenoic acid （DHA） glycerides from algae oil by freezing crystallization was studied. The fatty acid composition uct were determined by GC -MS. The optimal process conditions were obtained and content of the prod- as follows： with acetone as solvent, volume ratio of algae oil to solvent 1 ： 6, crystallization temperature - 40 ℃, crystallization time 8 h. Under the optimal conditions, the content of DPA in enriched algae oil increased from 11.65 % to 14.36% and the content of DHA improved from 44.64% to 57.47%.
出处
《中国油脂》
CAS
CSCD
北大核心
2015年第7期69-72,共4页
China Oils and Fats
基金
国家粮食公益性行业科研专项(201313007)
武汉轻工大学食品营养与安全研究院重大项目培育专项(2011z02)
关键词
藻油
DHA
DPA
甘油酯
冷冻结晶
algae oil
DHA
DPA
glyceride
freezing crystallization
