獐奶氨基酸和脂肪酸含量分析及奶胃微生物分离鉴定

Analysis of amino acid and fatty acid in the Hydropotes inermis milk and identification of the microorganism in the milk stomach

獐奶是幼獐的奶胃形成獐宝的基本物质,獐的奶胃是形成獐宝的主要场所。为进一步研究獐宝的体外合成机制,本文采用高效液相色谱和气相色谱法测定了獐奶中氨基酸和脂肪酸含量。并通过传统微生物分离手段对獐的奶胃微生物进行了分离鉴定。通过测定发现,獐奶中的水解氨基酸含量是牛奶的2.73倍。獐奶中丝氨酸、苏氨酸和半胱氨酸等游离氨基酸的含量高于牛奶中的含量,谷氨酸、天冬氨酸、甘氨酸和丙氨酸的含量却低于牛奶中的含量。獐奶中硬脂酸和亚油酸含量为牛奶的2倍多。通过对獐的奶胃微生物的分离和16S r DNA手段鉴定获得20种细菌,这些细菌主要为动物类乳杆菌、肠杆菌和肠球菌类,这些细菌对促进"獐宝"形成起一定的作用。

Hydropotesinermis milk can be synthesized stomach. In this study, the contents of amino acid investigated and compared by high performance to Hydropotesinermis swinhoe in the Hydropotesinermis milk and fatty acid in milk and Hydropotesinermis milk were iquid chromatography and gas chromatography, and the microorganism in the Hydropotesinermis milk stomach were also separated for exploring the synthesis mechanism of Hydropotesinermis swinhoe.The content of hydrolysis amino acid in HydropotesinermJs milk was 2.73 times more than milk.The contents of free amino acids （serine, threonine and cysteine）in Hydropotesinermis milk were more, but the free amino acids （glutamic acid, aspartic acid, glycine and alanine）were less than milk.The contents of stearic acid and linoleic acid in Hydropotesinermis milk were 2 times more than milk. In addition, 20 kinds of bacterium including animal Laetobacillus, Enterobacter and Enterococcus which play roles in the formation of Hydropotesinermls swinhoe were separated and indentified by 16S rDNA blasting.