碳、氮源对红曲黄色素色价和色调值的影响

Impacts of carbon and nitrogen sources on color value and tone value of yellow Monascus pigments

本文对红曲黄色素发酵条件进行了探究，红曲菌sjs-6发酵所得红曲黄色素，仅在420nm处有单吸收峰。不同碳源种类对色素产量有较大影响，对产色素种类影响不大；氮源对黄色素种类和产量均有较大影响，有机氮源可促使橙、红色素的产生，而无机氮源更有利于单产黄色素。发酵进程曲线显示，72～120h，菌体量处于稳定水平，是红曲菌sis-6产黄色素的关键时期，120h之后随着碳源耗尽，菌体自溶，产色素能力下降，色价保持稳定。通过正交实验可得，当以6％（w／v）玉米淀粉为碳源，0．4％（w／v）硫酸铵为氮源时，培养基初始pH为5．0，发酵温度为30℃时，发酵144h，黄色素色价可达378U／mL。产量是国内现有报道水平（100U／mL）的3倍多。由于菌株非突变株，具有较好的稳定性。由此，为实现红曲黄色素的工业化生产奠定了基础。

In this study,the fermentation conditions for the yellow pigments produced by submerged fermentation of Monascus sp.sjs-6 were investigated.It was found that the carbon sources varieties had large impact on the output of Monascus yellow pigments.However,not only the yield but also the kinds of pigment were affected by different nitrogen sources of culture medium.Organic nitrogen source could promote the production of orange, red pigment, but inorganic nitrogen source was good at the production of yellow pigment.Incubating at 30 ℃ for 6 days,a high yield（378 U/mL）of Monascus yellow pigments （MYP）was obtained in the optimum fermentation medium, in Which,6% （w/v）corn starch,0.4% （w/v） （NH4）2SO4 was used as the carbon, nitrogen source, respectively with initial pH of 5.0.Moreover,the MYP had single absorption peak at 420 nm which was different from the studies by other researchers.The yield of MYP presented in this research was more than 3 times over the other reports in our country.As it was a wild strain without mutant,the strain was relatively stable than the mutant strains reported by other researchers.So,this study would lay a foundation for the subsequent large scale production of MYP.