响应面法优化β-乳球蛋白粗提物自组装V_E的工艺条件

Optimization for the processing conditions of vitamin E combined by crude- extracted β- lactoglobulin using response surface methodology

为了优化β-乳球蛋白粗提物自组装VE工艺条件,通过单因素实验研究了β-Lg粗提物与VE质量比例、磁力作用时间、热激温度对β-Lg-VE结合工艺的影响。在单因素实验基础上,利用Design Expert.V8.0.6.1软件,设计了3因素、3水平的Box-Behnken中心组合实验,进行响应面法分析,优选β-乳球蛋白粗提物自组装VE工艺条件。结果表明,β-Lg粗提物自组装VE的最佳工艺条件:β-Lg粗提物与VE质量比例为19∶1,磁力作用时间49 min,热激温度为72℃。此条件下,β-Lg-VE的结合率为(68.5±0.98)%,与理论值68.98%拟合性较好。β-Lg-VE自组装分子的溶解性较好,其在食品工业中具有广泛应用。

To optimize the processing conditions of vitamin E combined by crude-extracted β-lactoglobulin, the effects of quality ratio,magnetic stirring time and heat-shock temperature on the combined process were studied by single factor test.And based on the single factor test, a Box-Behnken Center-combined experiment involving three factors and three levels was designed and analysed by response surface methodology.It was indicated that the best processing conditions of vitamin E combined by crude-extracted β-1actoglobulin were as follows：the quality ratio 19：1, Magnetic stirring time 49 min,heat-shock temperature 72℃.And under the conditions,the actual combined rate of vitamin E can reach up to（68.5 ＋0.98）% which was consistent with the theoretical combined rate 68.98% ,it was proved that the fit of the model was good and the above optimized processing conditions were stable and reliable.The solubility of β-Lg-VE was so great that it can be used widely in food industry.