表没食子儿茶素没食子酸酯(EGCG)抗氧化微乳液的制备与表征

Preparation and characterization of epigallocatechin-3- gallate( EGCG) antioxidant microemulsion

以肉豆蔻酸异丙酯为油相,吐温80-司盘80复配表面活性剂(HLB为6)为表面活性剂,乙醇为助表面活性剂(Km值为4∶1)制备了油包水型的表没食子儿茶素没食子酸酯(EGCG)抗氧化微乳液,并对其稳定性和抗氧化活性进行考察。通过测定电导率鉴别微乳液类型,纳米粒度仪测定微乳液的粒径,考察25℃条件下贮藏时间对微乳粒径的影响,评价微乳液的稳定性。同时,采用氧化稳定仪测定EGCG微乳液在花生油和亚麻籽油中的抗氧化活性。结果表明,微乳液外观澄清透明,在25℃贮藏条件下的稳定性好,放置50 d后微乳液的粒径变化小,放置期间未出现分层、破乳现象,并且在植物油中表现出较好的抗氧化活性。

EGCG water in oil（W/O） antioxidant microemulsion （ME） was prepared by use of isopropyl myristate as oil phase, tween 80 and span 80 （ HLB d） as surfactant, ethanol as cosurfactant （ Km 4： 1 ）, and its stability and antioxidant activity were studied.The type of ME was identified by measuring the electrical conductivity, and the stability of ME was evaluated through measuring the size of ME and the impact of storage time on the ME size under 2.5 ℃ condition.The antioxidant activity of ME was measured by Rancimat oxidative stability analyzer in peanut oil and flaxseed oil.The results showed that the appearance of the ME was clear and transparent,the ME size changed little and exhibited good stability when stored at 25 ℃ for 50 d, meanwhile, the ME had strong antioxidant activity in vegetable oil.