微囊化猕猴桃的便秘预防效果

Prophylactic effect of microencapsulated kiwi on constipation

本研究对微囊猕猴桃的活性炭诱导昆明小鼠便秘预防效果进行了研究。将微囊猕猴桃对昆明小鼠实施喂养9 d,对小鼠体重、粪便状态、排便时间、小肠推进率和胃动素(MTL)、胃泌素(Gas)、内皮素(ET)、生长抑素(SS)、乙酰胆碱酯酶(ACh E)、P物质(SP)、血管活性肠肽(VIP)血清水平进行了测定以评价微囊猕猴桃的便秘预防效果。微囊化猕猴桃饲料组的小鼠的排便较为正常,接近正常组和沙可啶处理组。10%微囊猕猴桃饲料喂养可以缩短小鼠排出首例黑便的时间(112 min),短于5%微囊猕猴桃饲料喂养小鼠。10%微囊猕猴桃饲料还可以使小鼠的小肠活性炭推进率达到77.7%,显著高于5%微囊猕猴桃饲料喂养组和对照组。同时相比对照组小鼠,微囊猕猴桃可以显著(p<0.05)提高小鼠血清中的MTL、Gas、ET、ACh E、SP、VIP水平,降低SS的水平。通过成分分析发现微囊猕猴桃含有丰富的维生素A、C、E和膳食纤维。这些结果表明微囊猕猴桃包含有丰富的功能性物质,具有预防小鼠便秘的效果。

This study was to investigate the prophylactic effect of microencapsulation kiwi fruit on activated carbon- induced constipation in Kunming mice.KM mice were fed with microencapsulation kiwi fruit for 9 d.Body weight, defecation status, defecation time, gastrointestinal transit, and the serum levels of MTL （ motilin）, Gas （ gastrin）, ET （ endothelin ）, SS （ somatostatin ）, AChE （ acetylcholinesterase ）, SP （ substance P ）, VlP （ vasoactive intestinal peptide） were used to evaluate the preventive effect of microencapsulation kiwi fruit on constipation. Bisacodyl, a laxative drug,was used as a positive control.After inducing constipation, the defecation status of mice fed with microencapsulation kiwi fruit was almost normal,was close to the normal mice and constipation therapeutic drug （bisacodyl） treated mice.tO% microencapsulation kiwi fruit could reduce the time to the first black stool defecation （! 12 min） ,which was shorter than 5% microencapsulation kiwi fruit treated mice.10% microencapsulation kiwi fruit could also increase the gastrointestinal transit 77.7%, which was significantly higher than 5% microencapsulation kiwi fruit and control group. In contrast to the control mice, the serum levels of MTL, Gas, ET, ACHE, SP and VlP were significantly （p 〈 0.05）increased and the serum levels of SS were reduced in the mice treated with microencapsulation kiwi fruit.Through the analysis of components, microencapsulation kiwi was rich in vitamin A, C, E and dietary fiber.These results demonstrated that microencapsulation kiwi included rich functional components, and it had preventive effect on mouse constipation.