双孢蘑菇原生质体融合子的特性与分子鉴定

Characteristics and molecular identification of protoplast fusants fromAgaricus bisporus

采用原生质体技术,以双孢蘑菇主推品种As2796和浙农1号为亲本,获得性状优异的融合子Z-3和Z-7,二者均可在34℃生长良好,菌丝纤维素酶活性和漆酶活力均比As2796高,酯酶同工酶谱显示与亲本差异明显,ITS序列证实二者与As2796同源性分别为99.7%,99.3%.栽培试验得出新菌株Z-3和Z-7的产量分别比As2796高12.5%和5.1%.从其经济性状的综合指标看,可考虑进一步的区域试验与示范推广栽培.

Two fusants, named Z-3 and Z-7, were screened out from protoplast fusion between two main cultivated strains Agaricus bisporus 2796 and Zhe Nong No. 1. Results showed both of them can grow at 34℃, the activities of cellulose enzyme and laccase were higher than As2796. Besides, it demonstra- ted big differences by analyzing EST isoenzyme banding patterns. Further ITS sequences homologies were identified with 99.7% and 99.3%, respectively. Cultivated experiment proves that the strains of Z-3 and Z-7 increased the yields with 12.5% and 5.1%, respectively. It can be promoted after further field experiments, regarding its good economic indicator.