黄瓜幼苗对外源氰化物耐受性研究

Putative mechanism of cucumber seedlings tolerance to exogenous cyanide

用不同浓度的氰化钾(KCN)溶液处理黄瓜幼苗,发现黄瓜幼苗可以耐受较高浓度(1mM)外源氰化钾胁迫的能力.氰化钾预处理明显诱导交替氧化途径并激活β-氰丙氨酸合成酶(β-CAS).研究结果表明,交替氧化途径和β-CAS对减轻呼吸抑制是必不可少的,可以提高植物对外源氰化物的耐受力并维持正常生长状态.

In this study, different concentration of potassium cyanide （KCN） had been used to treat cu- cumber seedlings and found that it had a good tolerance to higher concentration （lmM） of exogenous cy- anide treatment. Physiological analyses showed that cyanide pretreatment significantly induced the alter- native oxidase （AOX） pathway and activated theβ-cyanoalanine synthase （β-CAS）. The results indica- ted that the AOX pathway and β-CAS were essential to alleviate respiration inhibition and then contribu- ted to normal growth during plant response to exogenous cyanide.