免疫重建荷人食管癌SCID鼠肿瘤模型的建立

The establishment of human esophageal cancer model in Hu-PBL-SCID mice

目的 建立荷人食管癌重症联合免疫缺陷（SCID）鼠肿瘤模型,为食管癌的治疗提供一种能够模拟人免疫环境的荷瘤动物模型.方法 密度梯度离心分离健康志愿者外周血淋巴细胞（PBL）.将SICD小鼠15只用简单随机法分为实验组和对照组,实验组12只,对照组3只;实验组每只SCID鼠接受腹腔内注射约2×107个PBL,重建人的免疫系统,次日,每只鼠右后肢皮下注射约5×106个ECA109细胞,接种后观察肿瘤生长情况,于接种肿瘤细胞后第3、4、5、6周处死小鼠,称量脾重,制备肿瘤组织切片,检测鼠血清中IgG水平.结果 实验组SCID鼠成瘤率100％,移植瘤局限性生长,未见转移灶.实验组SCID鼠第3、4、5、6周脾重分别为（55.44±4.45）mg、（88.62±2.24） mg、（125.98±2.19）mg、（213.71±2.96） mg,与对照组的（41.87±2.97） mg相比,均增加,差异均具有统计学意义（第3周P＜ 0.05,第4、5、6周均P＜0.01）.实验组SCID鼠血清IgG含量第3、4、5、6周分别为（122.40±20.73） μg/ml、（149.09±18.90） μg/ml、（161.81 ±6.27） μg/ml、（174.62±17.45）μg/ml,较对照组的（0.20±0.01） μg/ml均增加（均P＜ 0.01）.结论 对SCID小鼠采用腹腔注射人PBL,皮下接种人食管癌ECA109细胞,可成功建立免疫重建荷人食管癌SCID小鼠模型.

Objective To establish a mouse model for human esophageal cancer.Methods Human PBL were isolated directly from whole blood by density gradient centrifugation.Fifteen SCID mice were randomly divided into two groups.Group A was control group,and in group B there were 12 mice intraperitoneally injected with 2×107 human PBL and subcutaneously injected with 5×106 ECA109 cells.The rate of tumor transplantation,tumor growth,metastasis and histological features were observed.After 3,4,5,6 weeks of engraftment,the level of IgG in mouse serum and the spleen weight were detected.Results The successful rate of tumor transplantation was 100 %.Metastasis was not found.After 3,4,5,6 weeks of engraftment,the spleen weight in group B were （55.44±4.45） mg,（88.62±2.24） mg,[（125.98±2.19） mg] （P 〈 0.05） and （213.71±2.96） mg,which had statistical significance compared with the control group （41.87±2.97） mg.The level of IgG was significantly higher than that in control group （P 〈 0.01）.Conclusion The experimental results demonstrate that human esophageal cancer models have been established in Hu-PBL-SCID mice.