高粱SbNAC0584基因克隆与表达分析

The Analysis on the Cloning and Expression of Sb NAC0584 Gene in Sorghum bicolor L.

NAC转录因子是植物体特有且在调控植物生长发育、抵抗逆境胁迫等方面具有重要生物功能的转录调控因子。本研究从高粱中克隆得到一个受逆境胁迫诱导表达的NAC家族基因,系统进化分析结果表明该基因与玉米Zma NAC0584亲缘关系最近,故将其命名为Sb NAC0584;Sb NAC0584基因全长929 bp,编码290个氨基酸,氨基酸序列比对结果表明Sb NAC0584蛋白N-末端具有典型的NAC保守结构域,C-末端为序列高度多样性的转录调控结构域。采用实时荧光定量PCR技术分析Sb NAC0584基因对不同非生物逆境胁迫的应答模式,结果表明Sb NAC0584受Na Cl、脱水、PEG胁迫和植物激素ABA诱导表达上调,推测Sb NAC0584在高粱非生物胁迫应答过程中发挥重要作用;组织特异性表达分析结果表明Sb NAC0584在高粱旗叶和根中表达量相对较高。本研究为深入研究Sb NAC0584的抗逆生物学功能奠定基础。

NAC proteins are plant-specific transcription factors that play important role in abiotic stress adaptations and tolerance, as well as in the plant processes in development. In this study, by searching against the plant genome databases, a stress induced NAC member was isolated from sorghum. It was designed as SbNAC0584 for its close relationship with maize ZmaNAC0584. The full-length cDNA of SbNAC0584 was 929 bp that encoded 290 amino acids. Sequence analysis indicated that a NAC conserved domain was localized in the N-terminal region of the SbNAC0584 protein. A highly diverse sequence was also observed in the C terminus. Transcription analyses were carried out to determine the effects of various abiotic stresses and of the phytohormone ABA on the expression of the SbNAC0584. SbNAC0584 was significantly induced by NaCl, dehydration, PEG and ABA treatments. We speculated that it might be involved in the response to abiotic stresses. The spatial expression pattern of SbNAC0584 revealed that higher transcripts existed in the root and flag leaf of sorghum plants than those in other tissues. This information provides a background for the further functional study of SbNAC0584.