叶黄素对砷致小鼠肝脏毒性的预防保护作用

Preventive effects of lutein on liver toxicity in mice induced by arsenic

目的观察提前给予小鼠叶黄素干预对砷肝脏毒性的预防效果。方法将84只SPF级昆明种小鼠,雌雄各半,随机分为35 d空白组、70 d空白组、35 d单纯叶黄素组、70 d单纯叶黄素组、砷染毒组、35 d叶黄素拮抗组和70 d叶黄素预防组,按叶黄素40 mg/kg,As2O35 mg/kg剂量灌胃给药。测定血清中谷氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性,肝脏匀浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)含量、总抗氧化能力(T-AOC)及一氧化氮(NO)含量,采用单因素方差分析对比组间差异。结果砷染毒组AST和ALT活性、MDA含量显著高于70 d空白组,SOD活性、GSH及NO含量、T-AOC水平显著低于70 d空白组(P<0.05);70 d叶黄素预防组AST及ALT活性、MDA含量低于35 d叶黄素拮抗组(P<0.05),SOD活性、NO及GSH含量、T-AOC水平均高于35 d叶黄素拮抗组(P<0.05)。结论提前给予叶黄素干预可以提高小鼠肝脏组织抗氧化损伤能力,从而拮抗砷的肝脏毒性作用。

Objective To explore the intervention effects of lutein on liver toxicity in mice induced by arsenic trioxide（ As2O3） and to evaluate the preventive effect and the antioxidant mechanism of lutein. Methods A total of 84 healthy KM mice were randomly divided into eight groups. There were 35 d control group,70 d control group,35 d lutein group,70 d lutein group,arsenic exposure group,lutein treatment group and lutein prevention group. The activity and level of AST,ALT,T-AOC,MDA,GSH,SOD,NO in liver tissue were measured by kits. Compares the difference between each group of the single factor analysis of variance. Results The activity of ALT,AST and the contents of MDA of 70 d control group were significantly lower than that arsenic exposure group. The contents of GSH,T-AOC,NO and the activity of SOD of 70 d control group were increased significantly than arsenic exposure group. The activity of ALT,AST and the contents of MDA of lutein prevention group were significantly lower than that lutein treatment group.The contents of GSH,T-AOC,NO and the activity of SOD of lutein prevention group were increased significantly than lutein treatment group. The differences were statistically significant（ P〈0. 05）. Conclusion Lutein can improve antioxidation function and has protective effect on liver injury of mice induced by arsenic.