红缘天牛对沙棘7种挥发物的EAG和嗅觉行为反应

EAG and olfactory behavioral responses of Asias halodendri to seven volatiles from Hippophae rhamnoidea

为了开发防治红缘天牛(Asias halodendri)的生物诱剂,研究了红缘天牛对不同化合物的反应.选取沙棘的7种挥发物成分,以单一组分和多组分混合配方作用于红缘天牛,观测其嗅觉行为和触角电位(EAG)反应.结果表明,7种单一成分中,除了戊醛、2-甲基丁醛和壬烷3种化合物外,2,3-丁二酮、正辛烷、正十一烷和正庚烷4种化合物对雌性和雄性红缘天牛均表现出不同程度的引诱活性.而且雌、雄虫均对一定体积分数的2,3-丁二酮和正辛烷具有较强的EAG和嗅觉行为反应.多组分配方实验中,11种配方对红缘天牛均具有引诱活性.雌虫对配方G的EAG相对值最高,为1.46;其次是配方C和E,EAG相对值均超过1.35,3种配方同对照组之间的差异均具有统计学意义(p<0.05).雄虫对配方C的EAG相对值最高,为1.45,同对照组之间的差异具有高度统计学意义(p<0.01);其次是配方B和J,EAG相对值分别为1.37和1.40;再次是配方G、H和K,5个配方同对照组之间的差异均具有统计学意义(p<0.05).11种配方中,配方C和G对雌、雄虫均有强烈的引诱作用.

In order to develop new biological attractants,the response of Asias halodendri to different compounds was inves tigated. Seven volatiles from Hippophae rhamnoidea in single component or mixed formulations were selected to study the Electroan-tennography（EAG） and smell behavioral responses of Asias halodendri. The results showed that,with the exception of three volatiles,i.e. valeraldehyde,2- methylbutylaldehyde and nonane,the other four kinds of volatiles,namely2,3-butyl diketone,octane,undecane and n-heptane,had attractant activities to Asias halodendri. Both male and femal had strong EAG and smell behavioral reaction to 2,3-butyl diketone and octane. Meanwhile,Asias halodendri could be attracted by all of the eleven mixed formulations. The femal had the highest EAG value（1.46） to G and higher values to C and E（〉1.35）. All the three formulations had obviously attractant activities than the control（p〈0.05）. However the male showed the highest EAG value（1.45）to C,which had significant attractant activities than the control（p〈0.01）. Then B（1.37）and J（1.40）had obviously attractant activities than the control（p〈0.05）,as well as G,H,K. Among the eleven formulations,C and G had fierce attractant activities to both male and femal.