长角血蜱Pmy蛋白在大肠杆菌中表达条件的优化

Optimization of expression conditions of Pmy protein from Haemaphysalis longicornis in E. coli

为提高长角血蜱副肌球蛋白(Pmy)的表达量,本研究对所在课题组前期构建的含有p ET-32(a+)-pmy的E.coli BL21载体的表达条件进行优化,考察温度、时间和异丙基硫代-β-D-半乳糖苷(IPTG)浓度对Pmy表达量的影响.SDS-PAGE电泳结果表明,最优表达条件为:诱导温度37℃,诱导时间8 h,IPTG浓度0.4 mmol/L.

To improve expression level of paramyosin（Pmy）from Haemaphysalis longicornis,the expression conditions of E. coli BL21 recombined with p ET-32（a＋）-pmy before in our laboratory were optimized. Three factors were involved in this study,including tempera-ture,time and concentration of isopropyl β-D-1-thiogalactopyranoside（IPTG）. The results of SDS-PAGE showed that the op timal temperature was 37 ℃,the inducing time was 8 h,and the concentration of IPTG was0.4 mmol /L.