干旱胁迫对导入高粱DNA的水稻变异系R21生理的影响

Effect of Drought Stress on Physiological Characters of Rice Variant Line R21 by Introgression Sorghum DNA

本研究选用导入高粱DNA的水稻变异系R21、受体缙恢1号及巴西陆稻为实验材料,采用裂区试验设计,分析在干旱胁迫下水稻变异系R21的生理特性。结果表明:经12 d干旱胁迫后,各品种的脯氨酸、SOD和POD活性及MDA含量均显著提高,叶绿素a、叶绿素b和总叶绿素含量及可溶性蛋白含量显著降低。耐旱品种如R21和巴西陆稻比不耐旱品种缙恢1号具有较高的脯氨酸含量、SOD活性和POD活性,以及较低的可溶性蛋白含量和MDA含量。受到12 d干旱胁迫后,耐旱品种R21和巴西陆稻的脯氨酸含量、SOD活性和POD活性上升幅度显著高于缙恢1号,可溶性蛋白含量下降幅度和MDA含量上升幅度显著小于缙恢1号。本研究对水稻耐旱品种培育具有一定实践指导意义。

In this study, the rico variant line R21 by introgression sorghum DNA, together with its receptor parent Jin Hui 1 and IAPAR9 were used as experimental materials to study the physiological characters under drought stress with split-plot experiment design. The results showed that： （i） The contents of proline, SOD, POD and MDA under the 12-day drought stress were significantly higher than that under normal conditions. While chlorophylla, chlorophyll b and total chlorophyll were signitlcandy different. （ii） Higher proline content, activi- ties of SOD and POD and lower contents of soluble protein and MDA existed in drought toleranco varieties, such as 1121 and IAPAR9, than that in Jin Hui 1, which indicated that the proline contents of drought-tolerant varieties enhanced significantly under drought treatment. （iii） Increasing range of SOD, POD activities and proline contents were higher significantly in 17,21 and IAPAR9 than in Jinhui 1. While increas- ing range of MDA contents and decreasing range of soluble protein in R21 and IAPAR.9 were significantly lower than that of Jinhuil. These results had some practical directive significance for rico drought tolerance varieties.