紫锥菊叶柄高效再生体系的建立

Establishment of An Efficient Regeneration System for Echinacea Purpurea Petiol

紫锥菊具有增强免疫和抗感染的作用,在国际上具有悠久的应用历史,近年来国内也有引种.该文以紫锥菊的不同器官(根、叶片、叶柄)为外植体,研究各激素配比对不同外植体建立再生体系的影响.结果表明:根、叶片、叶柄不定芽分化的适宜培养基分别为MS+0.5 mg/L 6-BA+0.2 mg/L NAA、MS+2.0 mg/L 6-BA、MS+0.5 mg/L6-BA,诱导率分别为43.8%、73.7%和94.8%;增殖的适宜培养基为MS+0.5 mg/L 6-BA,增殖系数为9.0,生根培养基为MS基本培养基.研究结果为紫锥菊的快速繁殖和进一步开展遗传转化奠定基础.

Echinacea Purpurea, which has the functions of enhancing immunity and anti-infection, has been used as a medicinal plant for a long history worldwide, and was introduced to China in recent years. In order to establish an efficient regeneration system of E. Purpurea, we studied the effect of concentrations of 6 - BA and NAA on the plant regeneration of different explants. The results showed that ： a, the optimum media for bud differentiation from petiol, leaf and root were MS ＋ 0. 5 mg/L 6 - BA ＋ 0. 2 mg/L NAA, MS ＋ 2.0 mg/L 6 - BA, and MS ＋ 0. 5 mg/L 6 - BA, respectively ; b, the optimum media for differentiation and rooting were MS ＋ 0.5 mg/L 6 - BA, MS, respectively; c, the multiplication coefficient reached to 9.0. This work provides a practical plant regeneration method for rapid propagation and genetic modification of E, Purpurea.