外周血血管内皮抑素和血管抑素检测在胰腺癌患者病程判断中的意义

Significance of serum endostatin and angiostatin detection in judging the course of pancreatic cancer

目的探讨外周血血管内皮抑素和血管抑素检测在胰腺癌患者病程判断中的意义。方法选取134例胰腺癌患者和50例健康对照，根据胰腺癌患者病情状况分为不可切除组（A组，n：56）和可切除组（B组，n=78），分别于胰腺癌患者入院后1d和可切除患者术后7d，以及正常对照均抽取晨起空腹肘静脉血，采用酶联免疫吸附试验。ELISA）法对所有标本中血管内皮抑素和血管抑素含量进行检测。结果胰腺癌患者血清血管内皮抑素和血管抑素含量均高于对照组，差异均具有统计学意义（P〈0．05）。A组患者术前血清血管内皮抑素和血管抑素含量均高于B组，B组患者术后7d血清血管内皮抑素和血管抑素含量均低于术前，差异均具有统计学意义（P〈0．05）。Pearson相关分析显示，胰腺癌患者血清血管内皮抑素含量与血管抑素含量呈正相关（r=0．530，P=0．000），Spearman相关分析显示，胰腺癌患者血清血管内皮抑素和血管抑素含量均与患者病程呈正相关（r=0．796和0．637，均P〈0．05）。胰腺癌患者血清血管内皮抑素含量与肿瘤大小有关（P〈0．05），血管抑素含量与肿瘤大小和血管侵犯有关（P〈0．05）。结论胰腺癌患者血清血管内皮抑素和血管抑素含量均出现升高，且随病情进展含量升高，与肿瘤生长和血管侵袭密切相关。

Objective To investigate the significance of serum endostatin and angiostatin detection in judging the course of pancreatic cancer. Methods A total of 134 cases of pancreatic cancer patients and 50 healthy controls were selected. All cases were divided into unresectable group（ group A, n = 56） and resectable group （ group B, n = 78 ） according to the status of patients with pancreatic cancer. Morning fasting venous blood samples were taken at the first day after hospitalization and seventh day after sur- gery. The contents of endostatin and angiostatin in the samples were detected by enzyme-linked immunosorbent assay （ ELISA method）. Results The levels of serum vascular endostatin and angiostatin in pa- tients with pancreatic cancer were significantly higher than the control group （ P ~ 0.05 ）. The levels of preoperative serum endostatin and angiostatin in group A were higher than group B, and the postoperative levels of serum endostatin and angiostatin in group B were significantly lower than the preoperative levels （ P 〈 0.05）. Pearson correlation analysis showed that the content of serum endostatin in patients with pan- creatic cancer was positively correlated with angiostatin（ r = O. 530, P = O. 000 ）. Spearman correlation a- nalysis showed that the course of disease was positively correlated with the levels of serum endostatin（ r = 0. 796, P 〈 0. 05 ） and angiostatin （ r = 0.637, P 〈 0.05 ） in patients with pancreatic cancer. The content of serum endostatin in pancreatic cancer was related to tumor size （ P 〈 0.05 ）, and the content of angiostatin was related to tumor size and vascular invasion（P 〈 0.05 ）. Conclusion The contents of serum endosta- tin and angiostatin in patients with pancreatic cancer increase with the progressing of the disease. They are closely associated with tumor growth and vascular invasion.