G蛋白耦联受体5通过上皮-间充质转化促进结肠肿瘤侵袭转移的临床研究

Lgr5 promote invasion and metastasis of colon cancer through epithelial-mesenchymal transition:a clinical research

目的分析G蛋白耦联受体5（Lgr5）在结肠癌侵袭转移过程中的作用及其意义。方法采用免疫组织化学法检测97例结肠癌组织标本及其对应正常结肠组织标本中Lgr5、E-cadherin、N-cadherin的表达情况。结果在结肠癌中，Lgr5在肿瘤组织中表达阳性率为55．7％（54／97），正常组织中Lgr5表达阳性率为5．2％（5／97）。Lgr5在肿瘤组织与正常组织中表达比较差异有统计学意义（χ^2=54．480，P〈0．05）。E—cadherin在肿瘤组织中表达阳性率为51．5％（50／97），正常组织中E-cadherin表达阳性率为87．6％（85／97）。E—cadherin在肿瘤组织与正常组织中表达差异具有统计学意义（χ^2=29．837，P〈0．05）。N—cadherin在肿瘤组织中表达阳性率为66％（64／97），正常组织中N-cadherin表达阳性率为15．5％（15／97），N—cadherin在肿瘤组织与正常组织中表达比较差异有统计学意义（χ^2=51．271，P〈0．05）。Lgr5的表达，结肠癌淋巴结有无转移比较差异有统计学意义（χ^2=11．178，P〈0．05），有无远处转移比较，差异有统计学意义（χ^2=7．900，P〈0．05）。Lgr5的表达和E-cadherin的表达呈负相关（r=-0．562，P〈0．05），和N-cadherin的表达呈正相关（r=0．458，P〈0．05）。结论Lgr5表达水平的改变能够影响结肠癌细胞的侵袭转移能力，Lgr5可能促进结肠肿瘤细胞EMT的发生，参与了结肠癌的侵袭转移。

Objective To study the roles and significance of Lgr5 in invasion and metastasis of colon cancer. Methods Immunohistochemisruy was employed to detect the expression of Lgr5, E-cadherin and N-cadherin in 97 samples of colon cancer tissues and normal tissues. Results The positive rate of Lgr5 expression was 55.7% （54/97） in colon cancer tissues and 5.2% （5/97） in normal tissues, and there was significant difference between groups （ χ^2 = 54. 480, P 〈 0.05 ）. The positive rate of E-cadherin ex- pression was 51.5% （50/97）in colon cancer tissues and 87.6% （85/97）in normal tissues, and there was significant difference between groups（ χ^2=29. 837, P 〈 0.05 ）. The positive rate of N-cadherin expression was 66% （64/97）in colon cancer tissues and 15.5 % （15/97）in normal tissues, and there was significant difference between groups（ χ^2 = 51. 271 ,P 〈 0. 05 ）. In colon cancer tissues, the expression of Lgr5 was correlated with lymph node metastasis （ χ^2 = 11. 178, P 〈 0. 05 ） and distant metastasis （ χ^2 = 7.900, P 〈0. 05 ）. The expression of Lgr5 protein was negatively correlated （ r = - 0.562, P 〈 0. 05 ） to the E-cadherin protein, but it was positively correlated to the N-cadherin protein （ r = 0. 458, P 〈 0.05 ）. Conclusion Lgr5 may participate in the occurrence of EMT, affect the expression of E -cadherin and N-cadherin, and may be related to the metastasis and progression of colon cancer.