蛋白水解肽-Fe^(2+)配合物对克氏原螯虾体内Cd^(2+)的脱除效果

Removal effect of cadmium from red swamp crayfish(Procambarus clarkii) by hydrolysis peptides-Fe^(2+) complexes

为探讨克氏原螯虾体内重金属Cd2+残留的脱除方法,采用基础饲料中添加制备的蛋白水解肽-Fe2+配合物(TPH-Fe2+)进行饲喂,检测螯虾不同组织器官中Cd2+含量变化情况。结果显示,染毒后螯虾不同器官中Cd2+富集量依次为内脏团>肠>鳃>腹部肌肉>螯足肌肉,且在0~12 d自然净化过程中,无显著性下降趋势。添加400、800和1 200 mg/kg TPH-Fe2+饲喂螯虾,内脏团、鳃、肠及腹部肌肉中Cd2+含量均有不同程度下降;饲喂12 d后,螯虾不同器官中Cd2+脱除率为肠>内脏团>鳃>腹部肌肉,脱除率分别为40.60%、36.13%、33.36%和10.15%;TPH-Fe2+对螯虾螯足肌肉中Cd2+含量无显著性影响。研究表明,蛋白水解肽-Fe2+配合物能有效脱除螯虾体内Cd2+残留,可作为一种螯虾养殖饲料添加剂在基础饲料中加以应用。

The purpose of this study was to investigate the effects of prepared hydrolysis peptides-Fe2 ＋complexes（ TPH-Fe^2 ＋） on the depuration of cadmium（ Cd^2 ＋） from red swamp crayfish（ P. clarkii） tissues.The results indicated that the concentration of Cd^2 ＋in crayfish tissues from high to low was visceral mass intestine〉 gill 〉abdomen muscle 〉cheliped muscle respectively,all of which were not significantly decreased during the 12 days of depuration. How ever,significant differences were observed in groups feeding by 400,800,and 1 200 mg / kg TPH-Fe^2 ＋added into basal feed after 12 day ＇s feeding. The Cd2 ＋concentrations in intestine,visceral mass,gill,and abdomen muscle were significantly decreased by 40. 60%,36. 13%,33. 36%,and 10. 15%,respectively. Nevertheless,the variables of Cd^2 ＋concentrations in heliped muscle treated with 400- 1 200 mg / L TPH-Fe^2 ＋show ed no significant differences from controls at any point during the experimental period. For these reasons,TPH-Fe2 ＋can be recommended as depuration agent in crayfish feed to decrease the Cd^2 ＋concentrations in tissues.