HPLC法测定桑椹配方颗粒中槲皮素的含量

Determination of quercetin in Mulberry Concentrate Granules by HPLC

目的建立用高效液相色谱法测定桑椹配方颗粒中槲皮素的含量的方法。方法色谱柱为Krcomasil C18（4.6 mm×200 mm,5μm）;流动相：甲醇-0.4%磷酸（43∶57）;流速：1.0 mL·min^-1;检测波长：360 nm。结果槲皮素进样量为0.003 424-0.017 12μg（r=0.999 7）范围内与线性关系良好;平均回收率为98.16%,RSD为1.6%（n=6）。结论该方法简便、准确、重复性好,可用于桑椹配方颗粒中槲皮素的测定。

Objective To establish an HPLC method for the determination of quercetin in Mulberry Concentrate Granules. Methods Quercetin was separated on Krcomasil C18（ 4. 6 mm × 200 mm,5 μm） column,the mobile phase was methanol-0. 4% phosphate（ 43∶57） with the flow rate of 1. 0 mL·min^-1and the detection wavelength was 360 nm. Results The linear range of quercetin was 0. 003 424 - 0. 017 12 μg（ r = 0. 999 7）. The average recovery was 98. 16% and the RSD was 1. 6%（ n = 6）. Conclusion The method was simple,accurate and reproducible,and it could be used for the determination of quercetin in Mulberry Concentrate Granules.