幽门螺杆菌ssDNA适配子的筛选及其应用

Screening and application of ssDNA aptamers of Helicobacter pylori

目的通过富集的配基系统进化技术(SELEX)筛选出与幽门螺杆菌(HP)菌体具有高亲和性适配子,并且利用其检测组织和活体内HP的感染。方法培养HP细菌,利用SELEX筛选获得HP菌体的适配子。在HP感染患者活检组织验证其结合。结果从第6轮筛选所得ssDNA中筛选出一条高结合力的适配子命名为HP1,将适配子HP1区别于其他筛选出的适配子,并检测其与幽门螺旋杆菌的亲和力。结论初步获得了针对HP菌体筛选出高亲和性适配子,为开发HP诊断试剂奠定了基础。

Objective To screen the high-affinity aptamers of Helicobacter pylori（HP）by systematic evolution of ligands by exponential enrichment（SELEX）and to apply this technique to detect HP infection in tissue and in vivo.Methods HP was cultured.SELEX was applied to conduct screening for obtaining theaptamers of HP.The aptamers were used to detect the HP infection in biopsies.Results The high affinity aptamer was screened from the6 th rounds selection,named HP1.The aptamer HP1 was used to distinguish the HP from non-HP and its binding force with HP was detected.Conclusion The high-affinity aptamers binding to HP is successfully selected from the initial ssDNA library,which lays a foundation for the development of HP infection diagnostic reagents.