rs1122608多态性与中国汉族人血脂水平的关联研究

Association study between rs1122608 and lipid levels in Chinese Han population

目的探讨rs1122608(G/T)位点多态性与中国汉族人群血脂水平的关联性。方法在中南医院体检中心收集高脂血症患者204例作为高脂血症组,健康者187例作为健康对照组。用全自动生化仪检测血脂水平,采用聚合酶链反应-高分辨率熔解曲线分析技术(PCR-HRM)检测rs1122608(G/T)多态性。比较高脂血症组和健康对照组间等位基因和基因型的分布差异,同时比较不同基因型亚组之间血脂各指标的差异。结果高脂血症组与健康对照组GG型、GT型和TT型频率比较,差异无统计学意义(P>0.05),基因型亚组间各血脂指标总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)差异也无统计学意义(P>0.05)。结论 rs1122608(G/T)多态性与中国汉族人群血脂水平无明显相关性。

Objective To study the association of rs1122608 single nucleotide polymorphism（SNP）and serum lipid levels in the Han population.Methods A total of 204 hyperlipidemia cases as the hyperlipidemia group and 187 healthy people as the healthy control group were recruited for the study.Serum lipid levels were detected by automatic biochemical analyzers.The rs1122608 polymorphism was determined by polymerase chain reaction（PCR）and high-resolution melting（HRM）.The genotype and allele frequency distributions were compared between the hyperlipidemia group and the healthy control group.Serum lipid levels among different genotype subgroups in the hyperlipidemia group and the healthy control were compared also.Results The differences in the genotypic and allelic frequencies between the the hyperlipidemia group and the healthy control had no statistical significance（P〉0.05）.And there were no significant differences in TG,TC,HDL-C and LDL-C levels among different genotype subgroups in the hyperlipidemia group and the healthy control.Conclusion The rs1122608 polymorphism is not associated with lipid levels in Chinese Han population.