夏枯草提取物对痤疮相关致病菌抑菌活性及机制研究

Antibacterial Activity and Mechanism of Extracts from Prunellae Spica Against Pathogenic Organisms of Acne

目的探讨夏枯草提取物对痤疮相关致病菌抑菌活性及作用机制,并初步锁定物质基础。方法通过测定3种夏枯草提取物对金黄色葡萄球菌(ATCC 29213)、表皮葡萄球菌(CMCC26069)和痤疮丙酸杆菌(ATCC6919)的抑菌活性、抑菌曲线、对金黄色葡萄球菌的细胞壁透性和离子渗漏的影响,阐述夏枯草提取物的抑菌活性及作用机制。结果 3种夏枯草提取物对金黄色葡萄球菌、表皮葡萄球菌和痤疮丙酸杆菌均有抑制作用,作用强度:水提取物>乙醇提取物>超声提取物。夏枯草提取物通过影响受试菌细胞壁透性,导致胞体K+、Mg2+的渗出而抑制细菌生长。结论夏枯草提取物对3种痤疮致病菌均有显著抑制作用;其抑菌机制与影响细胞壁和细胞膜的通透性有关。

Objective To study the antibacterial activity and mechanism of extracts from Prunellae Spica against pathogenic organisms of acne. Methods The study was performed by determining bacteriostatic curve of Staphylococcus aureus （S.aureus）, Staphylococcus epidermidis （S.epidermidis） and Propionibacterium acnes （P. acnes）, the permeability of cell wall, content of peptidoglycan and ion leakage of S. aureus. Results It was found that three extracts from Prunellae Spica could prolong lag phase, inhibit growth of S. aureus, S. epidermidis and P. acnes, significantly affect the permeability of cell wall and increase the efflux of potassium and magnesium ion of S. aureus. The antibacterial activi-ty intensity was shown as：water extract＆gt;ethanol extract〉ultrasonic extract. Conclusion Prunellae Spica possesses the good antibacterial activity against pathogenic organisms of acne. Its antibacterial mechanism is related to affecting the permeability of cell membrane and cell wall.