中央丘脑GABA_A受体在全身麻醉意识消失中作用的研究

Research on the Role of GABA_A Receptor in Central Thalamus in Unconsciousness Induced by General Anesthesia

目的探讨中央丘脑内GABAA受体在全身麻醉药导致意识消失中的作用。方法采用脑区微注射技术分别在丙泊酚麻醉前和麻醉中于中央丘脑给予GABAzine和相等容量的生理盐水,观察翻正反射消失时间和恢复时间,以及翻正反射消失率和微注射给药后体动反应的发生率。结果中央丘脑内注射GABAzine可显著延长大鼠翻正反射消失时间(P<0.05),并缩短翻正反射恢复时间(P<0.05);丙泊酚麻醉中于中央丘脑给予GABAzine可发生明显的体动反应(P<0.05)。结论于中央丘脑内微注射GABAA受体拮抗剂可逆转丙泊酚所导致的意识消失作用,说明中央丘脑内的GABAA受体是全身麻醉导致意识消失的重要靶点。

Objective To probe the role of GABA^ receptor in central thalamus in the unconsciousness induced by general anesthe- sia. Methods By means of intracranial microinjection, GABAzine and normal saline with equal volume were microinfused into central thalamus before and during propofol anesthesia respectively. Time to loss of right reflex （LORR）, incidence of LORR, time to emergence and incidence of body movement after microinfusion were observed. Results Microinfusion of GABAzine into central thalamus prolonged the time to LORR（P 〈 0.05 ）and decreased the time to emergence（ P 〈 O. 05 ）. Microinfusing GABAzine into central thalamus during propofol anesthesia induced significant body movements（P 〈 0.05）. Conclusion Microinfusion of GABAA receptor antagonist into cen- tral thalamus reversed the unconsciousness induced by propofol, indicating that GABAA receptor in central thalamus is an important target by which general anesthetics induce unconsciousness.