葛根素保护压力负荷诱导的心肌肥厚

Puerarin Protects Pressure Overload Induced Cardiac Hypertrophy

目的探讨葛根素在压力负荷诱导的心肌肥厚中的作用及其机制。方法雄性C57小鼠随机分为假手术组、葛根素治疗+手术组和单纯手术组。在胸主动脉缩窄术后1周开始喂药[小鼠饮食中加葛根素,给药剂量约65mg/(kg·d)持续12周,12周后取心脏,比较各组小鼠心重体重比、肺重体重比和心重胫骨长度比,同时比较各组小鼠的死亡率以评价葛根素对压力负荷诱导的心肌肥厚的影响。结果手术12周后葛根素组心重体重比、肺重体重比和心重胫骨长度比明显低于手术组,且葛根素治疗组小鼠死亡率明显下降,病理染色结果显示与手术组相比葛根素治疗组NF-κB核转位明显下降,提示葛根素可能通过NF-κB该信号通过减轻心肌肥厚。结论葛根素能减轻压力负荷诱导的心肌肥厚,其机制可能是通过抑制NF-κB信号通路发挥作用。

Objective Puerarin, main active ingredient in kudzu root, has been widely used in the treatment of cardiovascular dis- ease and diabetes in traditional Chinese medicine. Whilethe role of puerarin in cardiac hypertrophy remains unclear. To investigate the effect of puerarin in pressure overload - induced cardiac hypertrophy. Methods Aortic banding was performed to induce cardiac hypertro- phy. C57 male mice were randomly divided into sham operator group, Aortic bandingwith or without puerarin treatment. Mice were given puerarin after 1 weeks of aortic banding ,puerarin were added to the diet at a dosage of 65mg/（ kg d）. Heart was removed after 12 weeks of aortic banding to compare the heart weight/body weight, lung weight/body weight and heart weight/tibia length of each group as well as the mortality. Results The heart weight/body weight, lung weight/body weight and heart weight/tibia length were significant lower in pu- erarin group compared to vehicle group after 12 weeks of aortic handing. The mortality in puerarin group was also reduced after 12 weeks of aortic handing, when comparing to vehicle group. Pathological staining result showed that compared with vehiclegroup, nuclear translo- cation of NF - KB was significantly decreased in puerarin, suggesting that puerarin regulating cardiac hypertrophy may through the NF - kB signal. Conclusion Puerarin can inhibit the pressure overload induced cardiac hypertrophy, and the mechanism may be involved in theblockage of NF - KB signaling pathway.