HIV感染者血浆、尿液巨细胞病毒检测的临床分析

Clinical Analysis of Patients with Human Immunodeficiency Virus by Cytomegalovirus in Plasma and Urine

目的分析HIV感染者血浆、尿液中巨细胞病毒检出率的差异性及其临床意义。方法对2012年7月-2013年7月间在杭州市西溪医院住院的202例HIV感染者的血浆和尿液标本进行巨细胞病毒核酸检测，分析两种类型的标本检出巨细胞病毒的差异性，同时观察CD4＋T淋巴细胞计数与巨细胞感染的关系并分析其临床意义。结果202份血浆检出巨细胞病毒60例，检出率为29．70％（60／202）。202份尿液检出巨细胞病毒95例，检出率为40．03％（95／202），两种类型标本巨细胞病毒检出率差异有统计学意义（P〈0．05）。60例血浆中检出巨细胞病毒标本其CD4＋T淋巴细胞计数均〈250个／微升，其中CD4＋T淋巴细胞计数在0～100个／微升者35例占58．33％（35／60），在101～250个／微升者25例占41．67％（25／60）。结论HIV感染者血浆、尿液巨细胞病毒检测存在差异，血浆巨细胞病毒检测更具有临床意义。外周血CD4＋T淋巴细胞计数越低更易发生巨细胞病毒感染。

Objective To analyze the difference in the detection rates of cytomegalovirus between plasma and urine in patients with human immunodeficiency virus （HIV） infection and its clinical significance. Methods Cytomegalovirus nucleic acid of 202 cases of HIV infected patients hospitalized in Hangzhou Xixi Hospital from July 2012 to July 2013 were detected, and the difference in the detection rates of cytomegalovirus between two types of specimens were analyzed. In addition, the relationship between CD4 cell count and cytomeg- alovirus infection was also investigated. Results The detection rates of cytomegalovirus in plasma and urine specimens were 29.70% （60/202） and 40.03% （95/202）respectively. The difference in the detection rates of cytomegalovirus between two types of specimens was statistically significant（ P 〈 0.05 ）. Of the 60 cases of CMV positive plasma specimens, the CD4 cell count was less than 250, the patients with CD4 cell count ranging from 0 to 100 and from 101 to 250 accounted for 58.33% （35/60）and 41.67% （25/60）respectively. Con- clusion There is a difference in detection of HCMV - DNA between plasma and urine. Detecting the HCMV - DNA in plasma will have more clinical significance. Patients with lower CD4 cell count are more likely to get cytomegalovirus infection.