阿特拉津对斑马鱼外周红细胞微核和核异常的影响

Effect of Atrazine on Micronuclei and Nuclear Anomalies in Peripheral Blood Erythrocytes of Danio rerio

本文采用静态水质接触染毒法与微核测定法,研究4种不同浓度阿特拉津(0、0.5、5.0和10.0 mg/L)在不同暴露时间(1、3、6和9 d)对斑马鱼外周血红细胞微核与核异常的影响。结果显示,与对照组相比,阿特拉津处理组出现了较多微核、核质外凸与内凹、核固缩、核不均等缢缩等核异常现象;阿特拉津处理组的微核率和核异常率随阿特拉津暴露浓度的增加和时间的延长而逐渐上升。而丙酮对斑马鱼的微核和核异常无明显影响。上述结果说明,阿特拉津对斑马鱼具有潜在的遗传毒性,且毒性效应在一定的条件下随暴露浓度的增加和时间的延长而增强。

In this study, the toxic effects of atrazine were investigated by detecting micronu- clei and nuclear anomalies in peripheral red blood cells of Danio rerio. Four atrazine levels（0, 0.5.5.0 and 10.0 mg/L）were chosen for gradient. The peripheral red blood cells of Danio rerio were sampled on 1,3,6 and 9 days respectively. /he results showed that there were more micronuclei and some kinds of nuclear anomalies after exposure to four levels of atrazine than that of the control groups, including micronucleus, nucleus endo-vacuole, enucleated cell, karyopyknosis and unequal nuclear division. The results showed that atrazine had a potential genetic toxicity on Danio rerio. In general, the genotoxic effects of atrazine increased with the increasing level of exposure concentrations or dutaion under certain exposure conditions.