江西省2007年-2012年急性弛缓性麻痹病例监测分析

Analysis of the AFP cases monitoring in Jiangxi from 2007 to 2012

目的通过对江西省2007年-2012年急性弛缓性麻痹(AFP)病例实验室监测结果进行分析,为本省保持无脊髓灰质炎状态提供依据。方法采用L20B、RD细胞进行脊髓灰质炎病毒PV分离,脊髓灰质炎毒株通过微量中和实验分型后送至国家脊髓灰质炎实验室进行型内鉴定。结果 2007年-2012年共接收2 084份AFP病例粪便标本,其中有71份分离出脊髓灰质炎病毒、300份分离出非脊髓灰质炎肠道病毒(NPEV),分离率分别为3.41%、14.40%;分离出的脊髓灰质炎病毒中Ⅲ型脊髓灰质炎病毒的构成比最高,为51.19%;2010年-2012年脊髓灰质炎病毒的分离率显著低于2007年-2009年;分离出的脊髓灰质炎病毒均为疫苗相关株。结论江西省脊髓灰质炎病毒学监测处于较高水平,2007年-2012年急性弛缓性麻痹病例未发现脊髓灰质炎野病毒和VDPV,Ⅲ型脊髓灰质炎疫苗株病毒是分离株中的优势株,应继续加强监测。

Objective In order to provide accurate, reliable science evidences for Polio free status in Jiangxi, to analyze the lab monitoring results of the acute flaccid paralysis（AFP） case from 2007 to 2012. Methods Polio viruses were isolated by L20B cell and RD cell. The isolated polio strains were typed by microneutralization test and then sent to national polio laboratory for intratypie identification. Results We received 2 084 AFP stool specimens from 2007 to 2012. Polio viruses were isolated from 71 stool specimens and NPEV were isolated from 300 stool specimens withthe isolation rate of 3.41% and 14.40% , respectively. The constituent ratio of the isolated polio virus type Ⅲ was the highest （51. 19% ） ; the isolation rates from 2010 to 2012 were significantly lower than that from 2007 to 2009. All isolated polio virus were Sabin - like strains. Conclusion Polio virological surveillance in Jiangxi province was at a high level. No wild polio virus and VDPV was found in the AFP cases from 2007 to 2012 in Jiangxi province. Polio virus type Ⅲ was the dominant strain. We should continue to strengthen surveillance.