Toll样受体4信号激活对胃癌细胞系生物学行为的影响

Effect of Toll-like receptor 4 signaling activation on the biological behavior of gastric cancer cell lines

目的 探讨Toll样受体（TLR）信号激活对胃癌细胞系增殖、迁移及侵袭能力的影响.方法 选取5株人胃癌细胞系和1株永生化正常胃黏膜细胞系,利用RT-PCR技术检测10种TLR的RNA本底表达水平.根据TLR RNA本底表达水平,检测TLR4在6株细胞系中蛋白表达水平.根据6株细胞系中TLR4蛋白表达水平,选取BGC823和AGS细胞系检测TLR4信号激活对其生物学行为的影响.结果 与其他TLR比,TLR4在6株细胞系中均有较高的RNA表达水平.TLR4蛋白在BGC823细胞（1.41 ±0.05）和AGS细胞（1.22±0.05）中的相对表达水平显著高于另外4株细胞系（均P＜0.05）.TLR4信号激活后,BGC823和AGS细胞系的增殖、迁移和侵袭能力明显增加（均P＜0.05）.结论 TLR4可能通过增强胃癌细胞增殖、迁移及侵袭能力,参与胃癌的浸润转移.

Objective To investigate the effects of Toll like receptor （TLR） signaling activation on the malignant biological behavior of gastric cancer cell lines.Methods RT-PCR was employed to detect the basal expression of TLRs in the gastric cancer cell lines.According to the basal RNA expression,the proteic expression of TLR4 of the cell lines was examined using Western blot analysis.BGC823 and AGS cell lines were chosen for investigating the effects of TLR4 signaling activation on the biological behavior due to the high level of TLR4 proteic expression.Results TLR4 RNA expression was higher than any other TLRs in the gastric cancer cell lines.Higher relative proteic expression of TLR4 was observed in BGC823 （1.41 ± 0.05）and AGS（1.22 ± 0.05） （all P 〈 0.05）.Activation of the TLR4 signaling pathway by LPS increased proliferation,migration and invasion of BGC823 and AGS cell lines （all P 〈 0.05）.Conclusion TLR4 may participate in the metastasis of gastric cancer through promotes the malignant biological behavior of gastric cancer cell,and may be a potential therapeutic target.