摘要
目的探索非典型抗精神病药利培酮对大鼠脑区中脑源性神经生长因子(brain-derived neurotrophic factor,BDNF)及其受体酪氨酸激酶受体B(tyrosine kinase receptor,TrkB)、P75神经营养因子受体(P75neurotrophin receptor,P75NTR)的调控作用。方法将大鼠随机分为利培酮处理组(n=8)和对照组(n=8),利培酮处理组采用0.25mg/kg利培酮腹腔内注射,对照组采用等量安慰剂腹腔内注射,连续处理14d后处死大鼠。取大鼠左右前额叶皮质、左右颞叶皮质及海马,提取RNA并进行BDNF、TrκB和P75 NTR mRNA的实时荧光定量PCR分析。结果利培酮处理组大鼠的左右前额叶皮质、左右颞叶皮质及海马中BDNF及TrkB mRNA的表达水平较对照组增高(P<0.05),而利培酮处理组P75 NTR mRNA的表达与对照组间差异无统计学意义(P>0.05)。结论利培酮能够上调大鼠左右前额叶皮质、左右颞叶皮质及海马BDNF-TrkB信号通路,而不能改变上述脑区中P75 NTR mRNA的表达水平。
Objective To investigate the effect of risperidone on the expression of brain-derived neurotrophic factor（BDNF）and its receptors,tyrosine kinase receptor（TrkB）and P75 neurotrophin receptor（P75NTR）in rat brain.Methods Sixteen SD rats were divided into two groups（n=8for each group）.The rats in experimental group were treated with risperidone〔0.25mg/（kg·d）〕for 14 d,while the control group was given placebo.Total RNA sample in prefrontal cortex,temporal cortex and hippocampus was extracted,and the expression of BDNF,TrkBand P75 NTR mRNA were determined by quantitative real-time PCR.Results The treatment of risperidone significantly up-regulated the expressions of BDNF and TrkB in prefrontal cortex,temporal cortex and hippocampus,while the expression of P75 NTR was not significantly changed. Conclusion Risperidone upregulated BDNF-TrkB signaling,but not BDNF-P75 NTR signaling,which may be helpful for the further pharmacological study of risperidone.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2015年第4期528-532,610,共6页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金(No.81071089
No.81201042)
2013年度高等学校博士学科点专项科研基金(新教师类)(No.20130181120032)资助
