京尼平诱导兔眼巩膜胶原交联的有效性和安全性

Efficiency and safety of crosslinking of scleral collagen using genipin in rabbit

目的探索京尼平诱导兔眼巩膜胶原交联的有效性和安全性,为防治近视进展寻找有效的方法。方法 20只成年新西兰大白兔,按简单数字随机方法选一眼Tenon’s囊下注射0.5 mmol·L-1京尼平0.5 mL 2次和4次各10只,再按简单数字随机选对侧眼10只为对照眼;每组5只测试生物力学,另5只进行组织学检查。结果与对照眼比较,Tenon’s囊下注射2次和4次,巩膜的极限应力分别增加了95%和161%;同时20%应变时弹性模量分别增加了204%和260%,而极限应变则分别下降了13%和22%。京尼平没有引起巩膜、脉络膜和视网膜中的细胞凋亡,也没有损伤邻近组织角膜和视神经,治疗区域没有明显的颜色变化。结论京尼平诱导兔眼巩膜胶原交联安全有效,是一种潜在的防治近视进展的方法。

Objective To evaluate the efficiency and safety of crosslinking scleral collagen using genipin in rabbit,and develop new therapy for preventing the progression of myopia. Methods Twenty New Zeal rabbits were randomly divided into two groups（ n = 10） for sub-Tenon＇s injection of 0. 5 mmol·L- 1genipin for 2 times and 4 times,and the contralateral eyes were randomly selected 10 eyes as control. Five eyes in each group were for stress-strain measurements,and other 5 eyes were prepared for histological examination. Results Compared with untreated sclera strips,after sub-Tenon injecting with genipin for 2 times and 4 times,the ultimate stress of genipin crosslinked sclera increased by an amplitude of 95% and 161%. The Young＇s modulus at 20% strain increased by 204% and 260%,respectively. And ultimate strain decreased by13% and 22%. It did not cause apoptosis in scleral,choroid and retinal cells; and no side effects were found in the peripheral cornea and optic nerve. There had no visible discoloration around treated area. Conclusion Genipin-induced collagen crosslinking is effective and safe in rabbit,and is a potential agent to strengthen the weaken sclera to prevent progressive myopia.