金针菇单、双核菌丝差异表达基因分析

Comparison of gene expression patterns between the monokaryotic and dikaryotic mycelia of Flammulina velutipes

对金针菇Flammulina velutipes单核菌丝W23的菌丝体以及与L11质配后的双核菌丝H1123菌丝体进行了转录组测序,以本实验室已获得的W23基因组为参考基因组研究两样本间差异基因,并对这些差异基因进行了GO功能和Pathway显著性富集分析。差异基因分析显示,两个样本中共有显著性差异表达的基因3 504个,其中在双核菌丝中上调、下调的基因数分别为2 151和1 353个。研究发现差异表达基因含有很多的转录因子基因、蛋白激酶以及WD40 repeat-like蛋白。Gene Ontology(GO)功能分析结果表明,extracellular region和membrane-enclosed lumen条目下的差异基因全部为上调表达,而envelope下的差异基因全部为下调表达,以利于双核菌丝分裂时锁状联合的形成而便于核的迁移。Pathway功能富集分析结果表明,脂肪酸、氨基酸以及大部分糖类合成相关基因具有比较活跃的上调表达。说明双核菌丝主要进行营养物质的富集,为下一步在合适条件下分化成原基,进入生殖生长阶段储备物质基础。

Based on the reference genome of monokaryotic strain W23 of Flammulina velutipes obtained previously in our aboratory, comparison of gene expression patterns in the monokaryotic and dikaryotic mycelium revealed that 3 504 genes had differences in gene expression. 2 151 genes were up-regulated and 1 353 genes were down-regulated. These genes contained a number of transcription factors, protein kinases and WD40 repeat-like proteins. According to gene ontology （GO） cluster analyses, all the genes belong to the extracellular region. Genes belonging membrane-enclosed lumen item were up-regulated but those belonging to envelope item were down-regulated. This might be involved in the mechanism of the formation of clamp connection and nuclear migration. Pathway analyses revealed that genes involving with fatty acid, amino saccharide synthesis were up-regulated. This suggested that dikaryotic mycelium could store nutrition and primordium formation at suitable condition in next sexual development stage acid and most prepare for the