Influence of dietary taurine and housing density on oviduct function in laying hens

日粮中添加牛磺酸对不同饲养方式蛋鸡输卵管功能的影响（英文）

Experiments were conducted to study the effects of dietary taurine and housing density on oviduct function in laying hens. Green-shell laying hens were randomly assigned to a free range group and two caged groups, one with low-density and the other with high-density housing. Each group was further divided into control（C） and taurine treatment（T） groups. All hens were fed the same basic diet except that the T groups＇ diet was supplemented with 0.1% taurine. The experiment lasted 15 d. Survival rates, laying rates, daily feed consumption, and daily weight gain were recorded. Histological changes, inflammatory mediator levels, and oxidation and anti-oxidation levels were determined. The results show that dietary taurine supplementation and reduced housing density significantly attenuated pathophysiological changes in the oviduct. Nuclear factor-κB（NF-κB） DNA binding activity increased significantly in the high-density housing group compared with the two other housing groups and was reduced by taurine supplementation. Tumor necrosis factor-α（TNF-α） m RNA expression in the high-density and low-density C and T groups increased significantly. In the free range and low-density groups, dietary taurine significantly reduced the expression of TNF-α m RNA. Supplementation with taurine decreased interferon-γ（IFN-γ） m RNA expression significantly in the low-density groups. Interleukin 4（IL-4） m RNA expression was significantly higher in caged hens. IL-10 m RNA expression was higher in the high-density C group than in the free range and low-density C groups. Supplementation with taurine decreased IL-10 m RNA expression significantly in the high-density group and increased superoxide dismutase（SOD） activity in the free range hens. We conclude that taurine has important protective effects against oviduct damage. Reducing housing density also results in less oxidative stress, less inflammatory cell infiltration, and lower levels of inflammatory mediators in the oviduct. Therefore, both dietary taurine and reduced housing density can ameliorate oviduct injury, enhance oviduct health, and promote egg production in laying hens.

目的:本研究旨在调查饲喂牛磺酸和降低饲养密度对蛋鸡输卵管功能的影响。创新点:着眼于动物健康的动物福利,探讨在现代社会高密度饲养的笼养鸡伴随的许多健康问题。方法:在本研究中,绿壳蛋鸡被随机分为散养组、低密度组和高密度组,每组又被分为对照组和饲喂牛磺酸实验组。每个组别的蛋鸡日常饮食是一样的,除了实验组中添加0.1%的牛磺酸。15天后,无菌采集输卵管组织,并分析了组织病理学学上的变化、炎症介质水平、氧化和抗氧化水平等指标的变化。结论:这些数据表明牛磺酸可以保护输卵管组织,降低炎症介质水平、氧化水平以及增强抗氧化水平等。另外,散养和低密度饲养同样能降低氧化应激和输卵管炎症因子水平等。