脂多糖激活基质金属蛋白酶表达诱导MC3T3-E1细胞的增殖

Activated expression of matrix metalloproteinase is involved in the proliferation of MC3T3-E1 cells induced by lipopolysaccharide

背景:基质金属蛋白酶及其抑制剂是一种含Zn+的蛋白水解酶,参与了多种组织的细胞外基质降解与组织重塑过程。目的:观察基质金属蛋白酶在脂多糖诱导的MC3T3-E1细胞增殖中的参与作用。方法:MC3T3-E1细胞随机分为4组:对照组、脂多糖低剂量组(1μmol/L)、脂多糖中剂量组(10μmol/L)和脂多糖高剂量组(100μmol/L)。分析各组细胞的细胞增殖率,并检测基质金属蛋白酶2,3,9及基质金属蛋白酶组织抑制因子1,2的表达。结果与结论:脂多糖处理MC3T3细胞后细胞的增殖率明显增强,且具有时间依赖性和浓度依赖性,且处理后基质金属蛋白酶及其抑制剂的表达均明显增强,差异有显著性意义。结果提示,基质金属蛋白酶表达的增强参与了脂多糖诱导的MC3T3-E1细胞增殖过程。

BACKGROUND：Matrix metal oproteinases and their inhibitors are proteolytic enzymes contaning Zn＋, and involved in extracel ular matrix degradation and tissue remodeling of a variety of tissues. OBJECTIVE：To observe the effects of matrix metal oproteinases in the proliferation of MC3T3-E1 cel s induced by lipopolysaccharide. METHODS：MC3T3-E1 cel line was divided into four groups randomly：control group, low-dose lipopolysaccharide group （1μmol/L）, moderate-dose lipopolysaccharide group （10μmol/L）, and high-dose lipopolysaccharide group （100μmol/L）. The proliferation rate in each group was analyzed. Matrix metal oproteinase 2, 3, 9 and matrix metal oproteinase inhibitor 1 and 2 expressions were detected. RESULTS AND CONCLUSION：The proliferation rate was increased greatly after medication of lipopolysaccharide in time-dependent and concentration-dependent manners. Moreover, the expressions of matrix metal oproteinases and their inhibitors were apparently enhanced, and showed significant differences. Results indicate that the enhanced expressions of matrix metal oproteinases participated in the proliferation of MC3T3-E1 cel s induced by lipopolysaccharide.