富硒化对博落回有效成分抑菌效果的影响研究

Study on the antibacterial effect of active ingredient of se-enriched Macleya cordata

目的研究富硒化对博落回姚效成分抑菌效果的影响。方法以青霉素钠、生理盐水为对照,采用琼脂扩散纸片法检测各富硒化博落回组处理9种常见细菌的平均抑菌圈直径;以20%甲醇、生理盐水作为对照,用20%的甲醇将平均抑菌圈最小的富硒化博落回组,无硒处理博落回组和青霉素钠组分别稀释,使浓度均为0.48、0.24、0.12、0.06、0.03 mg/m L,采用琼脂平板稀释法测定其MIC,评价对其9种常见细菌的抑制作用。结果富硒化处理能大幅提高博落回生物碱的抑菌效果,100μg/m L亚硒酸钠处理组的平均抑菌圈最小(15.33 mm);富硒化博落回生物碱对大肠杆菌O1、大肠杆菌P22、都伯林大肠杆菌的最低抑菌浓度为从原来0.96 mg/m L降低至0.24 mg/m L;对鼠伤寒杆菌、猪霍乱杆菌的最低抑菌浓度为0.12 mg/m L;对金黄色葡萄球菌、猪链球菌的最低抑菌浓度为0.06 mg/m L;对枯草芽孢杆菌和李氏杆菌的最低抑菌浓度小于0.03 mg/m L。结论富硒化显著改善博落回的抑菌效果,为今后富硒博落回的开发利用,奠定了理论基础。

Objective To study on antibacterial effect of active ingredient of se-enriched Macleya cordata.Methods Penicillin sodium and saline as control, detected the average inhibition zone diameter of se-enriched Macleya cordata group （ containing 0.96 mg/mL sanguinarine） on 9 common bacteria with disc agar diffusion method;20% methanol and saline as control, se-enriched Macleya cordata group which the average inhibition zone diameter was minimum, without se-enriched Macleya cordata group and penicillin sodium were diluted with 20% methanol, made concentration of 0.48, 0.24,0.12,0.06, 0.03 mg/mL.minimal inhibitory concentration （ MIC） of each treatment group was determined by agar dilution method, evaluated antibacterial effect of se-enriched Macleaya cordata group.Results se-enriched Macleya cordata group could improve antibacterial effect significantly, the average inhibition zone of 100 μg/mL sodium selenite treatment group was minimum（Φ15.33 mm）;MIC of se-enriched Macleya cordata group on E.coli O1 , E.coli P22 , E.Dublin reduced from 0.96 to 0.24 mg/mL;MIC of S.typhimurium and S.suipare were 0.12 mg/mL;MIC of S.aureus and S.suis were 0.06 mg/mL; MIC of E.Dublin and L.murrayi were less than 0.03 mg/mL.Conclusion Se-enriched Macleya cordata improves the antibacterial effect signififcantly, and it lays the theoretical foundation for development and utilization of se-enriched Macleya cordata for future.